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. 2020 Mar 19;13(1):168.
doi: 10.1186/s13104-020-04999-2.

Transcriptional Response of Mar, Sox and Rob Regulon Against Concentration Gradient Carbapenem Stress Within Escherichia Coli Isolated From Hospital Acquired Infection

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Free PMC article

Transcriptional Response of Mar, Sox and Rob Regulon Against Concentration Gradient Carbapenem Stress Within Escherichia Coli Isolated From Hospital Acquired Infection

Shiela Chetri et al. BMC Res Notes. .
Free PMC article

Abstract

Objective: The present study was carried out to investigate the transcriptional response of marA (Multiple antibiotic resistance A gene), soxS (Superoxide S gene) and rob (Right-origin-binding gene) under carbapenem stress.

Results: 12 isolates were found over-expressing AcrAB-TolC efflux pump system and showed reduced expression of OmpF (Outer membrane porin) gene were selected for further study. Among them, over expression of marA and rob was observed in 7 isolates. Increasing pattern of expression of marA and rob against meropenem was observed. The clones of marA and rob showed reduced susceptibility towards carbapenems.

Keywords: Carbapenems; Escherichia coli; Real-time PCR; marA; rob; soxS.

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Expression of Mar, Sox and Rob of AcrAB overexpressing isolates under normal condition (without stress) relative Escherichia coli ATCC 25922
Fig. 2
Fig. 2
Expression of MarA (a), SoxS (b) and Rob (c) gene under carbapenem stress relative to Escherichia coli ATCC 25922
Fig. 3
Fig. 3
Sequence alignment of mutational pattern of marA sequence with the sequence of Escherichia coli ATCC 25922 strain

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References

    1. Duvaland V, Lister IM. MarA, SoxS and Rob of Escherichia coli—global regulators of multidrug resistance, virulence and stress response. Int J Biotech Well Indus. 2013;2(3):101–124. - PMC - PubMed
    1. Yang Q, Wang H, Sun H, Chen H, Xu Y, Chen M. Phenotypic and genotypic characterization of Enterobacteriaceae with decreased susceptibility to carbapenems: results from large hospital-based surveillance studies in China. Antimicrob Agents Chemother. 2010;54(1):573–577. doi: 10.1128/AAC.01099-09. - DOI - PMC - PubMed
    1. Oteo J, Delgado-Iribarren A, Vega D, Bautista V, Rodríguez MC, Velasco M, Saavedra JM, Pérez-Vázquez M, García-Cobos S, Martínez-Martínez L, Campos J. Emergence of imipenem resistance in clinical Escherichia coli during therapy. Int J Antimicrob Agents. 2008;32(6):534–537. doi: 10.1016/j.ijantimicag.2008.06.012. - DOI - PubMed
    1. Poirel L, Héritier C, Spicq C, Nordmann P. In vivo acquisition of high-level resistance to imipenem in Escherichia coli. J Clin Microbiol. 2004;42(8):3831–3833. doi: 10.1128/JCM.42.8.3831-3833.2004. - DOI - PMC - PubMed
    1. Stapleton PD, Shannon KP, French GL. Carbapenem resistance in Escherichia coli associated with plasmid-determined CMY-4 β-lactamase production and loss of an outer membrane protein. Antimicrob Agents Chemother. 1999;43(5):1206–1210. doi: 10.1128/AAC.43.5.1206. - DOI - PMC - PubMed

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