Human H1 histone gene promoter CCAAT box binding protein HiNF-B is a mosaic factor

Biochemistry. 1988 Aug 23;27(17):6534-41. doi: 10.1021/bi00417a051.


Vertebrate histone gene promoters in many cases contain an upstream element, 5'dCCAAT, that has been implicated in modulating the efficiency of transcription of a broad spectrum of genes. We have previously isolated a nuclear factor (HiNF-B) that binds specifically to the CCAAT element of a cell cycle regulated human H1 histone gene. This factor shows similarities with other CCAAT box binding proteins in that it recognizes the same sequence but shows a distinct chromatographic behavior. In the present study, we have employed the gel retardation assay to demonstrate that HiNF-B is a cell cycle independent DNA binding protein that is conserved in both human and mouse cells. Using a series of reconstitution experiments with partially purified HiNF-B fractions, we show that this factor requires association of at least two components for site-specific binding. The composite structure of HiNF-B suggests that binding of at least some CCAAT elements in vertebrates may require cooperative interaction of CCAAT box binding proteins with other factors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • CCAAT-Enhancer-Binding Proteins*
  • Cell Nucleus / metabolism
  • DNA Probes
  • DNA-Binding Proteins / metabolism*
  • Genes*
  • Genetic Vectors
  • Histones / genetics*
  • Humans
  • Mice
  • Molecular Sequence Data
  • Mosaicism*
  • Promoter Regions, Genetic*


  • CCAAT-Enhancer-Binding Proteins
  • DNA Probes
  • DNA-Binding Proteins
  • HiNF-B protein, human
  • HiNF-B protein, mouse
  • Histones