Effects of Litterfall on the Accumulation of Extracted Soil Humic Substances in Subalpine Forests

Xinyu Wei; Yulian Yang; Ya Shen; Zihao Chen; Yuliang Dong; Fuzhong Wu; Li Zhang

doi:10.3389/fpls.2020.00254

Effects of Litterfall on the Accumulation of Extracted Soil Humic Substances in Subalpine Forests

Front Plant Sci. 2020 Mar 5:11:254. doi: 10.3389/fpls.2020.00254. eCollection 2020.

Authors

Xinyu Wei^{1

2}, Yulian Yang^{2

3}, Ya Shen², Zihao Chen², Yuliang Dong², Fuzhong Wu^{1

2}, Li Zhang²

Affiliations

¹ Key Laboratory of Humid Subtropical Eco-geographical Process of Ministry of Education, Fujian Normal University, Fuzhou, China.
² Long-Term Research Station of Alpine Forest Ecosystems, Key Laboratory of Ecological Forestry Engineering, Institute of Ecology and Forestry, Sichuan Agricultural University, Chengdu, China.
³ Ecological Security and Protection Key Laboratory of Sichuan Province, Mianyang Normal University, Mianyang, China.

Abstract

Plant litter is one of the main sources of soil humus, but which can also promote primary humus degradation by increasing microbial activity due to the higher availability of energy released, resulting in a confusing relationship between litterfall and soil humus. Therefore, an in situ incubation experiment was carried out in three subalpine forests (coniferous, mixed and broadleaved forests) on the eastern Qinghai-Tibetan Plateau. We set up two treatments. One that allowed litterfall to enter the soil normally and the other prevented litterfall to enter the soil. Soils were sampled in October (the end of the growing season), January (the onset of the freezing season), March (the end of the freezing season), and May (the start of the growing season) from May 2017 to May 2018. By assessing the litterfall production, the content of total extracted humus, humic acid (HA) and fulvic acid (FA) in the topsoil (0-20 cm) in each incubation period, we determined the impact of litterfall on the content of humus extracted from the soil during the freezing and the growing season. Over 1-year incubation, soil total extracted humus and HA showed considerable decreases in the treatment of retained litterfall in the mixed forest but not in the coniferous or broadleaved forests. Moreover, litterfall significantly reduced the contents of soil total extracted humus and HA during the growing season in all three forests, while only reduced soil HA content in the broadleaved forest in the freezing season. The relationship between litterfall and soil extracted humic substances was greatly regulated by the seasonal dynamics of litter types and litter production in all forest types. The larger the amount of litterfall was, the more litterfall could promote the reduction of soil extracted humic substances. Compared with a single type of broadleaf or needle litter, mixed litterfall could promote a higher degradation of soil humic substances. However, broadleaf litter might lead to much greater decreases in soil humic substance than needle litter because it is more decomposable. These results indicate that the effect of litterfall on soil humic substances are mainly regulated by litter types and litter production. Moreover, the effects of litterfall on soil humic substances are more significant during the growing season than winter. This suggests that the longer growing season and a shorter winter caused by ongoing global warming may alter the relationships between litterfall and extracted humic substances, further disrupting the carbon balance of forest ecosystems in the subalpine forests.

Keywords: fulvic acid; humic acid; humic substances; litterfall; soil organic matter; subalpine forest.