Hoechst-tagged Fluorescein Diacetate for the Fluorescence Imaging-based Assessment of Stomatal Dynamics in Arabidopsis thaliana

Sci Rep. 2020 Mar 24;10(1):5333. doi: 10.1038/s41598-020-62239-w.


In plants, stomata regulate water loss through transpiration for plant growth and survival in response to various environmental stressors; and simple methods to assess stomatal dynamics are needed for physiological studies. Herein, we report a fluorescence-imaging-based method using fluorescein diacetate tagged with Hoechst 33342, a nuclear staining chemical probe (HoeAc2Fl) for the qualitative assessment of stomatal dynamics. In our method, the stomatal movement is inferred by simple monitoring of the fluorescence intensity in the nucleus of the stomata.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / metabolism
  • Arabidopsis Proteins / genetics
  • Carbon Dioxide / metabolism
  • Fluoresceins / chemistry*
  • Fluorescence
  • Fluorescent Dyes / chemistry
  • Gene Expression Regulation, Plant / genetics
  • Optical Imaging / methods*
  • Plant Leaves / metabolism
  • Plant Stomata / metabolism
  • Plant Stomata / physiology*
  • Plant Transpiration / physiology
  • Water / metabolism


  • Arabidopsis Proteins
  • Fluoresceins
  • Fluorescent Dyes
  • Water
  • Carbon Dioxide
  • diacetylfluorescein