In plants, stomata regulate water loss through transpiration for plant growth and survival in response to various environmental stressors; and simple methods to assess stomatal dynamics are needed for physiological studies. Herein, we report a fluorescence-imaging-based method using fluorescein diacetate tagged with Hoechst 33342, a nuclear staining chemical probe (HoeAc2Fl) for the qualitative assessment of stomatal dynamics. In our method, the stomatal movement is inferred by simple monitoring of the fluorescence intensity in the nucleus of the stomata.
Conflict of interest statement
The authors declare no competing interests.
Stomatal action directly feeds back on leaf turgor: new insights into the regulation of the plant water status from non-invasive pressure probe measurements.Plant J. 2010 Jun 1;62(6):1072-82. doi: 10.1111/j.1365-313X.2010.04213.x. Epub 2010 Mar 25. Plant J. 2010. PMID: 20345603
Cytokinin activity increases stomatal density and transpiration rate in tomato.J Exp Bot. 2016 Dec;67(22):6351-6362. doi: 10.1093/jxb/erw398. Epub 2016 Nov 2. J Exp Bot. 2016. PMID: 27811005 Free PMC article.
Natural variation in stomatal response to closing stimuli among Arabidopsis thaliana accessions after exposure to low VPD as a tool to recognize the mechanism of disturbed stomatal functioning.J Exp Bot. 2014 Dec;65(22):6529-42. doi: 10.1093/jxb/eru370. Epub 2014 Sep 9. J Exp Bot. 2014. PMID: 25205580 Free PMC article.
Putting the brakes on: abscisic acid as a central environmental regulator of stomatal development.New Phytol. 2014 Apr;202(2):376-91. doi: 10.1111/nph.12713. Epub 2014 Mar 10. New Phytol. 2014. PMID: 24611444 Review.
[Stomatal regulation of plants in response to drought stress].Ying Yong Sheng Tai Xue Bao. 2019 Dec;30(12):4333-4343. doi: 10.13287/j.1001-9332.201912.004. Ying Yong Sheng Tai Xue Bao. 2019. PMID: 31840480 Review. Chinese.