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. 2019 Feb 8;7(2):245-252.
doi: 10.1016/j.gendis.2019.02.001. eCollection 2020 Jun.

The Effects of Repeated Propofol Anesthesia on Spatial Memory and Long-Term Potentiation in Infant Rats Under Hypoxic Conditions

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Free PMC article

The Effects of Repeated Propofol Anesthesia on Spatial Memory and Long-Term Potentiation in Infant Rats Under Hypoxic Conditions

Mang Sun et al. Genes Dis. .
Free PMC article

Abstract

Propofol is widely used as an intravenous drug for induction and maintenance in general anesthesia. Hypoxemia is a common complication during perianesthesia. We want to know the effect of propofol on spatial memory and LTP (Long-term potentiation) under hypoxic conditions. In this study, 84 seven-day-old Sprague-Dawley rats were randomly assigned into six groups (n = 14)-four control groups: lipid emulsion solvent + 50% oxygen (CO), lipid emulsion solvent + room air (CA), lipid emulsion solvent + 18% oxygen (CH), and propofol + 50% oxygen (propofol-oxygen, PO); and two experiment groups: propofol + room air (propofol-air, PA), and propofol + 18% oxygen (propofol-hypoxia, PH). After receiving propofol (50 mg/kg) or the same volume of intralipid intraperitoneal (5.0 ml/kg), injected once per day for seven consecutive days, the rats were exposed to 18% oxygen, 50% oxygen and air, until recovery of the righting reflex. We found that the apoptotic index and activated caspase-3 increased in the PH group (P < 0.05) compared with the PA group, fEPSP (field excitatory postsynaptic) potential and success induction rate of LTP reduced in all propofol groups (P < 0.05). Compared with the PO group, the fEPSP and success induction rate of LTP reduced significantly in the PA and PH groups (P < 0.05). Moreover, compared with CH group, the average time of escape latency was longer, and the number of platform location crossings was significantly reduced in the PH group (P < 0.05). Thus, we believe that adequate oxygen is very important during propofol anesthesia.

Keywords: Anesthesia; Apoptosis; Hypoxemia; LTP; Propofol; Spatial memory.

Figures

Figure 1
Figure 1
Apoptotic index in each group — red arrows indicate TUNEL-positive neurons.
Figure 2
Figure 2
Caspase-3 protein expression in each group. Caspase-3 protein level was determined by immunohistochemistry.
Figure 3
Figure 3
fEPSP changes on P7 rat hippocampus in each group. A represents CO group, B represents CA group, C represents CH group, D represents PO group, E represents PA group, F represents PH group; 1: Before stimulation; 2: After stimulation.
Figure 4
Figure 4
The average time of escape latency in each group. *Represents P < 0.05, compared with control groups; #Represents P < 0.05, compared with PO group.
Figure 5
Figure 5
The number of platform location crossings in each group. *Represents P < 0.05 compared with PO group.

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