Lipidomics and RNA sequencing reveal a novel subpopulation of nanovesicle within extracellular matrix biomaterials

Sci Adv. 2020 Mar 20;6(12):eaay4361. doi: 10.1126/sciadv.aay4361. eCollection 2020 Mar.


Biomaterials composed of extracellular matrix (ECM) provide both mechanical support and a reservoir of constructive signaling molecules that promote functional tissue repair. Recently, matrix-bound nanovesicles (MBVs) have been reported as an integral component of ECM bioscaffolds. Although liquid-phase extracellular vesicles (EVs) have been the subject of intense investigation, their similarity to MBV is limited to size and shape. Liquid chromatography-mass spectrometry (LC-MS)-based lipidomics and redox lipidomics were used to conduct a detailed comparison of liquid-phase EV and MBV phospholipids. Combined with comprehensive RNA sequencing and bioinformatic analysis of the intravesicular cargo, we show that MBVs are a distinct and unique subpopulation of EV and a distinguishing feature of ECM-based biomaterials. The results begin to identify the differential biologic activities mediated by EV that are secreted by tissue-resident cells and deposited within the ECM.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Animals
  • Biocompatible Materials
  • Chromatography, Liquid
  • Extracellular Matrix
  • Extracellular Vesicles*
  • Fatty Acids / metabolism
  • Lipidomics* / methods
  • Liquid Phase Microextraction
  • Mice
  • Nanoparticles*
  • Phospholipids / metabolism
  • Sequence Analysis, RNA*
  • Subcellular Fractions


  • Biocompatible Materials
  • Fatty Acids
  • Phospholipids