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. 2020 Apr;51(2):191-197.
doi: 10.1007/s10735-020-09869-6. Epub 2020 Mar 26.

EGFR Inhibitor AG1478 Blocks the Formation of 3D Structures Mainly Through ERK Signaling Pathway in Matrigel-induced 3D Reconstruction of Eccrine Sweat Gland-Like Structures

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EGFR Inhibitor AG1478 Blocks the Formation of 3D Structures Mainly Through ERK Signaling Pathway in Matrigel-induced 3D Reconstruction of Eccrine Sweat Gland-Like Structures

Liyun Chen et al. J Mol Histol. .

Abstract

EGFR signaling plays important roles in the development of eccrine sweat glands. We previously demonstrate that Matrigel induces eccrine sweat gland cells to reconstruct the three-dimensional (3D) structures of eccrine sweat glands, but the mechanisms are still unknown. In the study, eccrine sweat gland cells were cultured within a 3D Matrigel, and EGFR inhibitor AG1478, or MEK1/2 inhibitor U0126, were added to the medium respectively. The morphology of the 3D-reconstructed eccrine sweat gland-like structures was observed, the localization of phospho-EGFR was detected, and protein levels of EGFR, phospho-EGFR, phospho-JAK, phospho-AKT and phospho-ERK were examined. The results showed that cells treatment with AG1478 from Day 0 of 3D cultures blocked formation of spheroid-like structures. AG1478 administration caused reduced phospho-EGFR, concomitant with downregulation of phospho-ERK1/2, but not phospho-JAK or phospho-AKT. Phospho-EGFR and phospho-ERK were reduced, and only a small number of 3D-structures were formed following treatment with U0126. We conclude that EGFR plays important roles in Matrigel-induced 3D structures of eccrine sweat gland-like structures, and ERK1/2 signaling is responsible, at least in part, for the effect of EGFR.

Keywords: ERK signaling pathway; Eccrine sweat glands; Epidermal growth factor receptor; Three-dimensional reconstruction.

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References

    1. Acta Histochem. 2017 Jul;119(6):620-623 - PubMed

References

    1. Dev Biol. 2001 Jan 1;229(1):163-75 - PubMed

References

    1. J Cell Physiol. 2007 Dec;213(3):649-53 - PubMed

References

    1. Cell Tissue Res. 2016 Jul;365(1):113-22 - PubMed

References

    1. J Mol Histol. 2017 Jun;48(3):219-223 - PubMed

References

    1. J Biol Chem. 2003 Jan 17;278(3):1824-30 - PubMed

References

    1. Cancers (Basel). 2018 Dec 26;11(1): - PubMed

References

    1. Nat Cell Biol. 2004 Feb;6(2):154-61 - PubMed

References

    1. J Biol Chem. 2001 Apr 27;276(17):14443-50 - PubMed

References

    1. Cell Tissue Res. 2013 Dec;354(3):897-902 - PubMed

References

    1. Cell Death Dis. 2017 Oct 26;8(10):e3147 - PubMed

References

    1. Histochem J. 1997 Oct;29(10):735-44 - PubMed

References

    1. Prog Histochem Cytochem. 2002;37(4):323-86 - PubMed

References

    1. J Mol Histol. 2016 Oct;47(5):485-90 - PubMed

References

    1. Int J Mol Sci. 2013 Sep 25;14(10):19361-84 - PubMed

References

    1. J Invest Dermatol. 1984 Nov;83(5):385-93 - PubMed

References

    1. Bioessays. 1995 Oct;17(10):839-46 - PubMed

References

    1. Nat Cell Biol. 2002 Apr;4(4):E75-6 - PubMed

References

    1. Dev Biol. 2000 Apr 15;220(2):183-96 - PubMed

References

    1. Exp Eye Res. 2017 Oct;163:58-63 - PubMed

References

    1. Cancer Cell. 2015 Apr 13;27(4):533-46 - PubMed

References

    1. Exp Dermatol. 2013 Oct;22(10):667-9 - PubMed

References

    1. J Histochem Cytochem. 2001 May;49(5):597-602 - PubMed

References

    1. Mar Drugs. 2019 Aug 10;17(8): - PubMed

References

    1. Exp Cell Res. 2015 Mar 1;332(1):67-77 - PubMed

References

    1. Biochem J. 2009 Mar 15;418(3):491-506 - PubMed

References

    1. J Clin Invest. 1996 Jun 1;97(11):2426-32 - PubMed

References

    1. Differentiation. 2009 Mar;77(3):298-306 - PubMed

References

    1. Adv Mater. 2020 Jan;32(3):e1805818 - PubMed

References

    1. J Biomed Mater Res A. 2019 Mar;107(3):663-677 - PubMed

References

    1. Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5455-60 - PubMed

References

    1. J Biol Chem. 2017 Aug 11;292(32):13391-13401 - PubMed

References

    1. Development. 1999 Jan;126(2):335-44 - PubMed

References

    1. Exp Dermatol. 2019 Jan;28(1):53-58 - PubMed

References

    1. J Mol Histol. 2018 Dec;49(6):567-575 - PubMed

References

    1. J Cell Physiol. 2019 Dec;234(12):23608-23617 - PubMed

References

    1. PLoS One. 2014 Nov 13;9(11):e112922 - PubMed

References

    1. Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14821-6 - PubMed

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