Determination of the capsular polysaccharide structure of the Klebsiella pneumoniae ST512 representative strain KPB-1 and assignments of the glycosyltransferases functions

Int J Biol Macromol. 2020 Jul 15;155:315-323. doi: 10.1016/j.ijbiomac.2020.03.196. Epub 2020 Mar 26.


Klebsiella pneumoniae strain KPB-1 was isolated in early 2011 from the pleural fluid of an inpatient admitted at an Italian hospital. It was characterized to produce the KPC-3 carbapenemase and to belong to sequence type 512, a derivative of sequence type 258 clade II characterized by the cps-2 gene cluster. The K-antigen of K. pneumoniae KPB-1 was purified and its structure determined by using GLC-MS of appropriate carbohydrate derivatives and 1D and 2D NMR spectroscopy of the native polysaccharide. All the collected data demonstrated the following repeating unit for the K. pneumoniae KPB-1 capsular polysaccharide: The reactions catalysed by each glycosyltransferase in the cps-2 gene cluster were assigned on the basis of structural homology with other Klebsiella K antigens.

Keywords: Capsular polysaccharide structure; Glycosyltransferases; Klebsiella pneumoniae KPB-1; NMR.

MeSH terms

  • Bacterial Capsules / chemistry*
  • Bacterial Proteins / economics
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Glycosyltransferases / genetics
  • Glycosyltransferases / metabolism*
  • Klebsiella pneumoniae / chemistry
  • Klebsiella pneumoniae / enzymology*
  • Klebsiella pneumoniae / genetics
  • Multigene Family
  • Polysaccharides, Bacterial / chemistry*
  • beta-Lactamases / economics
  • beta-Lactamases / metabolism*


  • Bacterial Proteins
  • Polysaccharides, Bacterial
  • Glycosyltransferases
  • beta-Lactamases
  • carbapenemase