Metabolic acetylation of phenelzine in rats

Res Commun Chem Pathol Pharmacol. 1988 Dec;62(3):397-406.


1-Acetyl-2-(2-phenylethyl)hydrazine (N2-acetylphenelzine) is identified as an acetylated metabolite of phenelzine in the rat. One hour after intraperitoneal administration of a high dose of phenelzine sulfate to rats, the blood and brain of the animals were extracted and analyzed by combined gas chromatography/electron impact mass spectrometry in the total ion and selected ion modes. This procedure provided unequivocal proof of the presence of N2-acetylphenelzine in these tissues. The other possible monoacetylated metabolite of phenelzine, 1-acetyl-1-(2-phenylethyl)hydrazine (N1-acetylphenelzine), and the diacetylated derivative, 1,2-diacetyl-2-(2-phenylethyl)hydrazine, were sought, but were not detected.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Animals
  • Brain / metabolism
  • Gas Chromatography-Mass Spectrometry
  • Male
  • Phenelzine / administration & dosage
  • Phenelzine / analogs & derivatives
  • Phenelzine / metabolism*
  • Rats
  • Rats, Inbred Strains


  • N-acetylphenelzine
  • Phenelzine