Reduced Serum Cholesterol and Triglyceride Levels in a Choline-Deficient L-Amino Acid-Defined High-Fat Diet (CDAHFD)-Induced Mouse Model of Non-alcoholic Steatohepatitis (NASH)

Biol Pharm Bull. 2020;43(4):616-618. doi: 10.1248/bpb.b19-00338.

Abstract

Non-alcoholic fatty liver disease (NAFLD) or non-alcoholic seatohepatitis (NASH) is one of the major health problems world wide, because of increased abdominal obesity. To date, specific and effective medications to treat or prevent NAFLD/NASH have not been established. To identify appropriate molecular targets for that purpose, suitable animal models of NAFLD/NASH have been explored. A choline-deficient amino acid-defined high fat diet (CDAHFD)-induced mouse model of NASH has been developed. However, its relevance to human NASH, including serum lipid profiles, have not been clearly defined. In this study, we have revealed that mice fed CDAHFD showed significantly lowerd serum total cholesterol and triglyceride (TG) levels, in addition to reduced body weight (BW). Furthermore, hepatic microsomal triglyceride transfer protein (MTP) expression was significantly downregulated in CDAHFD-fed mice. Thus, the current CDAHFD-fed mouse model has points that are distinct from human NAFLD/NASH, in general, which is based upon abdominal obesity.

Keywords: CD36; microsomal triglyceride transfer protein; serum lipid; steatohepatitis.

MeSH terms

  • Amino Acids
  • Animals
  • CD36 Antigens / genetics
  • Cholesterol / blood*
  • Choline
  • Choline Deficiency
  • Diet, High-Fat
  • Disease Models, Animal
  • Gene Expression
  • Liver / metabolism
  • Male
  • Membrane Transport Proteins / genetics
  • Mice, Inbred C57BL
  • Non-alcoholic Fatty Liver Disease / blood*
  • Non-alcoholic Fatty Liver Disease / genetics
  • RNA, Messenger / metabolism
  • Triglycerides / blood*
  • Tumor Necrosis Factor-alpha / genetics

Substances

  • Amino Acids
  • CD36 Antigens
  • Cd36 protein, mouse
  • Laptm4a protein, mouse
  • Membrane Transport Proteins
  • RNA, Messenger
  • Tnf protein, mouse
  • Triglycerides
  • Tumor Necrosis Factor-alpha
  • Cholesterol
  • Choline