Acinetobacter pullorum sp. nov., Isolated from Chicken Meat

J Microbiol Biotechnol. 2020 Apr 28;30(4):526-532. doi: 10.4014/jmb.2002.02033.

Abstract

A bacterial strain, designated B301T and isolated from raw chicken meat obtained from a local market in Korea, was characterized and identified using a polyphasic taxonomic approach. Cells were gram-negative, non-motile, obligate-aerobic coccobacilli that were catalase-positive and oxidase-negative. The optimum growth conditions were 30°C, pH 7.0, and 0% NaCl in tryptic soy broth. Colonies were round, convex, smooth, and cream-colored on tryptic soy agar. Strain B301T has a genome size of 3,102,684 bp, with 2,840 protein-coding genes and 102 RNA genes. The 16S rRNA gene analysis revealed that strain B301T belongs to the genus Acinetobacter and shares highest sequence similarity (97.12%) with A. celticus ANC 4603T and A. sichuanensis WCHAc060041T. The average nucleotide identity and digital DNA-DNA hybridization values for closely related species were below the cutoff values for species delineation (95-96% and 70%, respectively). The DNA G+C content of strain B301T was 37.0%. The major respiratory quinone was Q-9, and the cellular fatty acids were primarily summed feature 3 (C16:1 ω6c/C16:1 ω7c), C16:0, and C18:1 ω9c. The major polar lipids were phosphatidylethanolamine, diphosphatidyl-glycerol, phosphatidylglycerol, and phosphatidyl-serine. The antimicrobial resistance profile of strain B301T revealed the absence of antibiotic-resistance genes. Susceptibility to a wide range of antimicrobials, including imipenem, minocycline, ampicillin, and tetracycline, was also observed. The results of the phenotypic, chemotaxonomic, and phylogenetic analyses indicate that strain B301T represents a novel species of the genus Acinetobacter, for which the name Acinetobacter pullorum sp. nov. is proposed. The type strain is B301T (=KACC 21653T = JCM 33942T).

Keywords: Acinetobacter pullorum sp. nov.; antimicrobial resistance; chicken meat; taxonomy.

MeSH terms

  • Acinetobacter / classification*
  • Acinetobacter / cytology
  • Acinetobacter / drug effects
  • Acinetobacter / physiology
  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Base Composition
  • Chickens
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Genes, Bacterial
  • Genome, Bacterial
  • Microbial Sensitivity Tests
  • Nucleic Acid Hybridization
  • Phospholipids / chemistry
  • Phylogeny*
  • Poultry / microbiology*
  • Quinones / chemistry
  • RNA, Ribosomal, 16S / genetics
  • Republic of Korea
  • Sequence Analysis, DNA

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • Fatty Acids
  • Phospholipids
  • Quinones
  • RNA, Ribosomal, 16S