RNA-binding proteins (RBPs) comprise a large class of over 2,000 proteins that interact with transcripts in all manner of RNA-driven processes. The structures and mechanisms that RBPs use to bind and regulate RNA are incredibly diverse. In this review, we take a look at the components of protein-RNA interaction, from the molecular level to multi-component interaction. We first summarize what is known about protein-RNA molecular interactions based on analyses of solved structures. We additionally describe software currently available for predicting protein-RNA interaction and other resources useful for the study of RBPs. We then review the structure and function of seventeen known RNA-binding domains and analyze the hydrogen bonds adopted by protein-RNA structures on a domain-by-domain basis. We conclude with a summary of the higher-level mechanisms that regulate protein-RNA interactions.
Keywords: (∗)Insert Keywords Here(∗).
Copyright © 2020 Elsevier Inc. All rights reserved.
Conflict of interest statement
Declaration of Interests G.W.Y. is co-founder, member of the board of directors, equity holder, and paid consultant for Locana and Eclipse BioInnovations. G.W.Y. is a Distinguished Visiting Professor at the National University of Singapore. The terms of this arrangement have been reviewed and approved by the University of California, San Diego in accordance with its conflict of interest policies. All other authors declare no competing interests.
Macromolecular crowding: chemistry and physics meet biology (Ascona, Switzerland, 10-14 June 2012).Phys Biol. 2013 Aug;10(4):040301. doi: 10.1088/1478-3975/10/4/040301. Epub 2013 Aug 2. Phys Biol. 2013. PMID: 23912807
Quantitative proteomic analysis reveals concurrent RNA-protein interactions and identifies new RNA-binding proteins in Saccharomyces cerevisiae.Genome Res. 2013 Jun;23(6):1028-38. doi: 10.1101/gr.153031.112. Epub 2013 May 1. Genome Res. 2013. PMID: 23636942 Free PMC article.
SPOT-Seq-RNA: predicting protein-RNA complex structure and RNA-binding function by fold recognition and binding affinity prediction.Methods Mol Biol. 2014;1137:119-30. doi: 10.1007/978-1-4939-0366-5_9. Methods Mol Biol. 2014. PMID: 24573478 Free PMC article.
Diverse roles of RNA-binding proteins in cancer traits and their implications in gastrointestinal cancers.Wiley Interdiscip Rev RNA. 2019 May;10(3):e1520. doi: 10.1002/wrna.1520. Epub 2018 Nov 26. Wiley Interdiscip Rev RNA. 2019. PMID: 30479000 Review.
Unconventional RNA-binding proteins step into the virus-host battlefront.Wiley Interdiscip Rev RNA. 2018 Nov;9(6):e1498. doi: 10.1002/wrna.1498. Epub 2018 Aug 9. Wiley Interdiscip Rev RNA. 2018. PMID: 30091184 Free PMC article. Review.