A Group of Complexes Based on PAMAM and Quantum Dots Used in Clinical Immunoassays

Yang Liu; Lu Hou; Qiong Guo; Mingjin Zhang; Wei Shi

doi:10.1186/s11671-020-3291-5

A Group of Complexes Based on PAMAM and Quantum Dots Used in Clinical Immunoassays

Nanoscale Res Lett. 2020 Apr 3;15(1):71. doi: 10.1186/s11671-020-3291-5.

Authors

Yang Liu^{1

2}, Lu Hou^{1

2}, Qiong Guo^{1

2}, Mingjin Zhang^{1

2}, Wei Shi^{3

4}

Affiliations

¹ Key Laboratory for Molecular Enzymology & Engineering, the Ministry of Education, Jilin University, Changchun, 130012, Jilin, China.
² College of life sciences, Jilin University, Changchun, 130012, Jilin, China.
³ Key Laboratory for Molecular Enzymology & Engineering, the Ministry of Education, Jilin University, Changchun, 130012, Jilin, China. shiw@jlu.edu.cn.
⁴ College of life sciences, Jilin University, Changchun, 130012, Jilin, China. shiw@jlu.edu.cn.

Abstract

We report a group of complexes used in clinical immunoassays. The complexes include a PAMAM-conjugated goat anti-rabbit IgG and a QDs-conjugated goat anti-mouse IgG. When rabbit anti-antigen and mouse anti-antigen are added, the corresponding antigen will be detected. The experiment, using the complexes, is simple, convenient, short in time, and short in steps. It is also applicable to different experiment methods, like to be used with FCM (flow cytometry), ICC (immunocytochemistry), and IHC (immunohistochemistry) to detect many kinds of antigens.

Keywords: Clinical immunoassays; FCM; PAMAM; Quantum dots.

Abstract

Grant support