Complete genome analysis of a Staphylococcus aureus phage (vBSM-A1)

Arch Microbiol. 2020 Sep;202(7):1617-1626. doi: 10.1007/s00203-020-01867-2. Epub 2020 Apr 6.

Abstract

In this study, the genome of a new strain of lytic Staphylococcus aureus Herelleviridae, vBSM-A1, was characterized and annotated. The phage was isolated from sewage samples collected in Xinjiang Province, China. The genome of vBSM-A1 was found to comprise a linear double-stranded DNA of 140,654 bp length, with a G + C content of 30.33%. A total of 215 ORFs were detected in the phage DNA, 74 of which were functionally assigned. The 3D structure model of endolysin LysK (ORF 143) was created using Phyre2.

Keywords: 3D structure model; Genome analysis; Staphylococcus aureus.

MeSH terms

  • Base Composition
  • China
  • DNA, Viral / chemistry
  • DNA, Viral / genetics
  • Endopeptidases / chemistry
  • Endopeptidases / genetics
  • Genome, Viral / genetics*
  • Models, Molecular
  • Open Reading Frames
  • Protein Structure, Tertiary
  • Sequence Analysis, DNA
  • Staphylococcus Phages / genetics*
  • Staphylococcus Phages / isolation & purification

Substances

  • DNA, Viral
  • Endopeptidases
  • endolysin