Condensin I subunit Cap-G is essential for proper gene expression during the maturation of post-mitotic neurons

Elife. 2020 Apr 7:9:e55159. doi: 10.7554/eLife.55159.


Condensin complexes are essential for mitotic chromosome assembly and segregation during cell divisions, however, little is known about their functions in post-mitotic cells. Here we report a role for the condensin I subunit Cap-G in Drosophila neurons. We show that, despite not requiring condensin for mitotic chromosome compaction, post-mitotic neurons express Cap-G. Knockdown of Cap-G specifically in neurons (from their birth onwards) results in developmental arrest, behavioural defects, and dramatic gene expression changes, including reduced expression of a subset of neuronal genes and aberrant expression of genes that are not normally expressed in the developing brain. Knockdown of Cap-G in mature neurons results in similar phenotypes but to a lesser degree. Furthermore, we see dynamic binding of Cap-G at distinct loci in progenitor cells and differentiated neurons. Therefore, Cap-G is essential for proper gene expression in neurons and plays an important role during the early stages of neuronal development.

Keywords: D. melanogaster; DamID; condensin; development; developmental biology; neuron; post-mitotic.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / genetics
  • Adenosine Triphosphatases / metabolism*
  • Animals
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Drosophila Proteins / genetics*
  • Drosophila Proteins / metabolism
  • Drosophila melanogaster / cytology*
  • Drosophila melanogaster / genetics*
  • Female
  • Gene Expression Regulation, Developmental
  • Gene Knockout Techniques
  • Male
  • Mitosis / genetics*
  • Multiprotein Complexes / genetics
  • Multiprotein Complexes / metabolism*
  • Neurons / cytology*


  • DNA-Binding Proteins
  • Drosophila Proteins
  • Multiprotein Complexes
  • condensin complexes
  • Adenosine Triphosphatases

Associated data

  • GEO/GSE142112
  • GEO/GSE77860