Comparison study of the response with botulinum toxin muscle injection in the ICR mice from three different sources

Min-Soo Seo; Young-In Kim; Kyung-Ku Kang; Se-Kyung Oh; Soo-Eun Sung; Young-Suk Jung; Joon Yong Cho; HyunKeun Song; Dae Youn Hwang; Sang-Joon Park; Kil Soo Kim

doi:10.1186/s42826-019-0010-4

Comparison study of the response with botulinum toxin muscle injection in the ICR mice from three different sources

Lab Anim Res. 2019 Jul 26:35:11. doi: 10.1186/s42826-019-0010-4. eCollection 2019.

Authors

Min-Soo Seo^#¹, Young-In Kim^#², Kyung-Ku Kang¹, Se-Kyung Oh¹, Soo-Eun Sung¹, Young-Suk Jung³, Joon Yong Cho⁴, HyunKeun Song⁵, Dae Youn Hwang⁶, Sang-Joon Park⁷, Kil Soo Kim^{1

8}

Affiliations

¹ 1Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu, Korea.
² KPC Coporation, Gwangju, 12773 Korea.
³ 3Department of Pharmacy, College of Pharmacy, Pusan National University, Busan, Korea.
⁴ 4Department of Health and Exercise Science, Korea National Sport University, 88-15 Oryung-dong, Songpa-gu, Seoul, Korea.
⁵ Central Research Institute, Kine sciences, F1, Milovany, 28, Inchon-ro, Seongbuk-gu, Seoul, Korea.
⁶ 6Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, Korea.
⁷ 7Laboratory of Histology, College of Veterinary Medicine, Kyungpook National University, Daegu, Korea.
⁸ 8College of Veterinary Medicine, Kyungpook National University, Daegu, Korea.

^# Contributed equally.

Abstract

Botulinum-toxin A (BoNT/A) is a widely used not only for cosmetics but also for various experimental purposes including muscle-related research. In this study, we applied BoNT/A to mouse muscle of three different sources to compare and evaluate the biological and pathological response. The three different mouse sources consist of Korl:ICR (Korea FDA source), A:ICR (USA source) and B:ICR (Japan source) which were purchased from each different vendors. To compare the responses of ICR mice with BoNT/A muscle injection, we examined the body weight, hematological and serum biochemistry analysis. Also, we evaluated the muscle change by histopathological analysis and gene expression patterns of muscle-related target by qPCR. The body weight gain was decreased in the BoNT/A-treated group compared with the control group. In clinical pathologic analysis and gene expression patterns, the data showed that the responses in the BoNT/A-treated group were similar compared with the control group. Decreased muscle fiber was observed in BoNT/A-treated group compared with control group, while Korl:ICR showed a little low response with the other mouse sources. In conclusion, our results suggest that three different sources ICR mice (Korl:ICR, A:ICR and B:ICR) have a similar biological and pathological responses in BoNT/A muscle injection.

Keywords: Botulinum toxin; ICR mouse; Korl:ICR; Muscle.