Infection and Rapid Transmission of SARS-CoV-2 in Ferrets

Cell Host Microbe. 2020 May 13;27(5):704-709.e2. doi: 10.1016/j.chom.2020.03.023. Epub 2020 Apr 6.

Abstract

The outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in China and rapidly spread worldwide. To prevent SARS-CoV-2 dissemination, understanding the in vivo characteristics of SARS-CoV-2 is a high priority. We report a ferret model of SARS-CoV-2 infection and transmission that recapitulates aspects of human disease. SARS-CoV-2-infected ferrets exhibit elevated body temperatures and virus replication. Although fatalities were not observed, SARS-CoV-2-infected ferrets shed virus in nasal washes, saliva, urine, and feces up to 8 days post-infection. At 2 days post-contact, SARS-CoV-2 was detected in all naive direct contact ferrets. Furthermore, a few naive indirect contact ferrets were positive for viral RNA, suggesting airborne transmission. Viral antigens were detected in nasal turbinate, trachea, lungs, and intestine with acute bronchiolitis present in infected lungs. Thus, ferrets represent an infection and transmission animal model of COVID-19 that may facilitate development of SARS-CoV-2 therapeutics and vaccines.

Keywords: 2019-nCoV; 2019-novel coronavirus; COVID-19; SARS-CoV-2; ferrets; novel coronavirus disease; severe acute respiratory syndrome coronavirus 2; transmission; virus shedding.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / immunology
  • Betacoronavirus / immunology
  • Coronavirus Infections / pathology*
  • Coronavirus Infections / transmission*
  • Disease Models, Animal
  • Ferrets*
  • Pandemics
  • Pneumonia, Viral / pathology*
  • Pneumonia, Viral / transmission*
  • Viral Vaccines / immunology
  • Virus Shedding

Substances

  • Antibodies, Viral
  • Viral Vaccines

Supplementary concepts

  • COVID-19
  • severe acute respiratory syndrome coronavirus 2