Mitochondrial impairment and cytotoxicity effects induced by the marine epibenthic dinoflagellate Coolia malayensis

Ana T Varela; Raquel A F Neves; Silvia M Nascimento; Paulo J Oliveira; Miguel A Pardal; Elsa T Rodrigues; António J Moreno

doi:10.1016/j.etap.2020.103379

Mitochondrial impairment and cytotoxicity effects induced by the marine epibenthic dinoflagellate Coolia malayensis

Environ Toxicol Pharmacol. 2020 Jul:77:103379. doi: 10.1016/j.etap.2020.103379. Epub 2020 Mar 30.

Authors

Ana T Varela¹, Raquel A F Neves², Silvia M Nascimento³, Paulo J Oliveira⁴, Miguel A Pardal⁵, Elsa T Rodrigues⁶, António J Moreno⁷

Affiliations

¹ CFE-Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456, Coimbra, Portugal. Electronic address: anabio@ci.uc.pt.
² CFE-Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456, Coimbra, Portugal; Laboratory of Marine Microalgae, Department of Ecology and Marine Resources, Federal University of the State of Rio de Janeiro (UNIRIO), Av Pasteur 458-314B, 22290-240, Rio de Janeiro, Brazil. Electronic address: raquel.neves@uc.pt.
³ Laboratory of Marine Microalgae, Department of Ecology and Marine Resources, Federal University of the State of Rio de Janeiro (UNIRIO), Av Pasteur 458-314B, 22290-240, Rio de Janeiro, Brazil. Electronic address: silvia.nascimento@unirio.br.
⁴ CNC-Centre for Neuroscience and Cell Biology, UC Biotech, University of Coimbra, Lot 8A, Biocant Park, 3060-197, Cantanhede, Portugal. Electronic address: pauloliv@cnc.uc.pt.
⁵ CFE-Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456, Coimbra, Portugal. Electronic address: mpardal@uc.pt.
⁶ CFE-Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456, Coimbra, Portugal. Electronic address: etrodrig@uc.pt.
⁷ CNC-Centre for Neuroscience and Cell Biology, UC Biotech, University of Coimbra, Lot 8A, Biocant Park, 3060-197, Cantanhede, Portugal; Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456, Coimbra, Portugal. Electronic address: moreno@ci.uc.pt.

PMID: 32259764
DOI: 10.1016/j.etap.2020.103379

Abstract

Mitochondria was used to clarify the effects of Coolia malayensis strain UNR-02 crude extract by studying mitochondrial membrane potential (ΔΨ_m) generation and the fluctuations of ΔΨ_m associated with the induction of mitochondrial permeability transition (MPT). The cytoxicity of C. malayensis was also determined using both HepG2 and H9c2(2-1) cells. C. malayensis extract significantly depressed the oxidative phosphorylation efficiency, as was inferred from the perturbations in ΔΨ_m and in the phosphorylative cycle induced by ADP. Increased susceptibility to Ca²⁺-induced MPT was also observed. At the cellular level, the extract significantly decreased cell mass of both cell lines.

Keywords: Coolia malayensis; Cytotoxicity; Marine dinoflagellates; Mitochondrial toxicity assessment; Toxins.

MeSH terms

Animals
Cell Line
Cell Survival / drug effects
Complex Mixtures / toxicity*
Dinoflagellida*
Humans
Male
Membrane Potential, Mitochondrial / drug effects
Mitochondria, Liver / drug effects*
Mitochondria, Liver / physiology
Rats

Substances

Complex Mixtures