Protective Effect of Palm Oil-Derived Tocotrienol-Rich Fraction Against Retinal Neurodegenerative Changes in Rats with Streptozotocin-Induced Diabetic Retinopathy

Biomolecules. 2020 Apr 5;10(4):556. doi: 10.3390/biom10040556.


: Oxidative stress plays an important role in retinal neurodegeneration and angiogenesis associated with diabetes. In this study, we investigated the effect of the tocotrienol-rich fraction (TRF), a potent antioxidant, against diabetes-induced changes in retinal layer thickness (RLT), retinal cell count (RCC), retinal cell apoptosis, and retinal expression of vascular endothelial growth factor (VEGF) in rats. Additionally, the efficacy of TRF after administration by two different routes was compared. The diabetes was induced in Sprague-Dawley rats by intraperitoneal injection of streptozotocin. Subsequently, diabetic rats received either oral or topical treatment with vehicle or TRF. Additionally, a group of non-diabetic rats was included with either oral or topical treatment with a vehicle. After 12 weeks of the treatment period, rats were euthanized, and retinas were collected for measurement of RLT, RCC, retinal cell apoptosis, and VEGF expression. RLT and RCC in the ganglion cell layer were reduced in all diabetic groups compared to control groups (p < 0.01). However, at the end of the experimental period, oral TRF-treated rats showed a significantly greater RLT compared to topical TRF-treated rats. A similar observation was made for retinal cell apoptosis and VEGF expression. In conclusion, oral TRF supplementation protects against retinal degenerative changes and an increase in VEGF expression in rats with streptozotocin-induced diabetic retinopathy. Similar effects were not observed after topical administration of TRF.

Keywords: angiogenesis; apoptosis; diabetic retinopathy; streptozotocin; tocotrienol-rich fraction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Body Weight / drug effects
  • Cytoprotection / drug effects
  • Diabetic Retinopathy / chemically induced
  • Diabetic Retinopathy / pathology
  • Diabetic Retinopathy / prevention & control*
  • Gene Expression Regulation / drug effects
  • Male
  • Palm Oil / chemistry*
  • Rats
  • Rats, Sprague-Dawley
  • Retina / drug effects*
  • Retina / pathology*
  • Streptozocin / pharmacology*
  • Tocotrienols / chemistry*
  • Tocotrienols / pharmacology*
  • Vascular Endothelial Growth Factor A / metabolism


  • Tocotrienols
  • Vascular Endothelial Growth Factor A
  • Palm Oil
  • Streptozocin