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. 2020 Apr 7;1-3.
doi: 10.1038/s41423-020-0424-9. Online ahead of print.

SARS-CoV-2 Infects T Lymphocytes Through Its Spike Protein-Mediated Membrane Fusion

Free PMC article

SARS-CoV-2 Infects T Lymphocytes Through Its Spike Protein-Mediated Membrane Fusion

Xinling Wang et al. Cell Mol Immunol. .
Free PMC article

Conflict of interest statement

The authors declare no competing interests.


Fig. 1
Fig. 1
Sensitivity of T lymphocytes to SARS-CoV-2 infection. a Infection of pseudotyped SARS-CoV-2 and SARS-CoV. 293T/ACE2 and Huh7 are permissive cells, while HeLa is a nonpermissive cell line. b Expression of ACE2 mRNA in T cells. 293T/ACE2 cells used as a control. c Infection of pseudotyped SARS-CoV-2 and SARS-CoV on T lymphoid cell lines. d Inhibition of EK1 peptide on pseudotyped SARS-CoV-2 on MT-2 cells. e SARS-CoV-2 S-mediated cell–cell fusion on MT-2 cells. MT-2 cells were cocultured with 293T/SARS-CoV-2/EGFP cells. Cell–cell fusion was photographed under an optical microscope with fluorescence or visible light. Fused cells were indicated with a white arrow. Scale bars 800 μm. f Detection of SARS-CoV-2 NP-positive cells with flow cytometry. MT-2 cells were infected with SARS-CoV-2 at 1TCID50 per cell, respectively. Cells were fixed and permeabilized at 24 and 48 h post infection and immunolabeled for detection of SARS-CoV-2 NP. g Infection of MT-2 cells by SARS-CoV-2 is abortive. Cells were inoculated with SARS-CoV-2 at 1TCID50 per cell and harvested at 1, 6, 24, and 48 h post infection. Cell lysate (above) and supernatant (below) were collected to detect the viral N gene with RT-qPCR. In panels, bars and error bars represent means and standard deviations. Statistical analyses were performed using the unpaired t test. *p < 0.05

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