Cloning, expression and comparison of the properties of Scy p 9, a Scylla paramamosain allergen

Food Funct. 2020 Apr 30;11(4):3006-3019. doi: 10.1039/d0fo00004c.

Abstract

This study investigated the properties of Scy p 9 in mud crab (Scylla paramamosain). The gene sequence of filamin C obtained from crabs, which was denoted as Scy p 9, contains a 2544 bp open reading frame and encodes 848 amino acid residues. Recombinant Scy p 9 (rScy p 9) is expressed in Escherichia coli, which exhibits tertiary structure changes, and the IgE binding activity of rScy p 9 is higher than that of native Scy p 9 (nScy p 9). Moreover, this study explored the possibility of the presence and cross-reactivity of filamin C in 8 shellfish. IgE-specific binding to nScy p 9 and rScy p 9 in patients allergic to shellfish revealed that rScy p 9 was more sensitive than nScy p 9. The gene sequence of filamin C fills in the blank in shellfish. This study contributes to the understanding of the properties of Scy p 9, and the results indicate that rScy p 9 can be used as a candidate for component-resolved diagnosis in shellfish.

MeSH terms

  • Adolescent
  • Adult
  • Allergens / genetics*
  • Allergens / immunology
  • Allergens / metabolism
  • Animals
  • Brachyura / genetics*
  • Brachyura / immunology
  • Brachyura / metabolism
  • Child
  • Child, Preschool
  • Cloning, Molecular
  • Escherichia coli / metabolism
  • Female
  • Food Hypersensitivity / blood
  • Food Hypersensitivity / immunology*
  • Humans
  • Male
  • Middle Aged
  • Open Reading Frames
  • Shellfish*
  • Young Adult

Substances

  • Allergens