Differentiation Paths of Peyer's Patch LysoDCs Are Linked to Sampling Site Positioning, Migration, and T Cell Priming

Cell Rep. 2020 Apr 7;31(1):107479. doi: 10.1016/j.celrep.2020.03.043.

Abstract

The monocyte-derived phagocytes termed LysoDCs are hallmarks of Peyer's patches, where their main function is to sample intestinal microorganisms. Here, we study their differentiation pathways in relation with their sampling, migratory, and T cell-priming abilities. Among four identified LysoDC differentiation stages displaying similar phagocytic activity, one is located in follicles, and the others reside in subepithelial domes (SED), where they proliferate and mature as they get closer to the epithelium. Mature LysoDCs but not macrophages express a gene set in common with conventional dendritic cells and prime naive helper T cells in vitro. At steady state, they do not migrate into naive T cell-enriched interfollicular regions (IFRs), but upon stimulation, they express the chemokine receptor CCR7 and migrate from SED to the IFR periphery, where they strongly interact with proliferative immune cells. Finally, we show that LysoDCs populate human Peyer's patches, strengthening their interest as targets for modulating intestinal immunity.

Keywords: LysoDC; Peyer’s patches; antigen sampling; cell differentiation; dendritic cells; intestinal immunity; monocyte-derived cells; naive T cell priming; phagocytes; single-cell RNA sequencing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation / immunology*
  • Cell Movement / immunology
  • Dendritic Cells / immunology
  • Female
  • Humans
  • Intestinal Mucosa / metabolism
  • Macrophages / immunology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Middle Aged
  • Monocytes / immunology
  • Peyer's Patches / cytology*
  • Phagocytes / cytology*
  • Phagocytes / metabolism
  • T-Lymphocytes / immunology