Deleterious effects of exposure to electromagnetic radiation on public health have been widely studied. This study was conducted to evaluate the protective effect of vitamin supplementation (E or E + C) on passive avoidance learning (PAL) and memory in rats subjected to 900 MHz radiofrequency waves (RFW). Thirty adult male Sprague-Dawley rats (190 ± 20 g) were randomly divided into six groups as: control I (vehicle), control II (vitamin E 250 mg/kg), control III (vitamin E 100 mg/kg + l-ascorbic acid 200 mg/kg), and three exposed groups to RFW as: sham-exposed, treatment I (vitamin E), and treatment II (vitamin E + C). The duration of exposure was 30 continuous days (4 h/day). The PAL was evaluated on the last day by the shuttle box. Learning and memory of animals demonstrated as the duration of remaining within the light area, which is called the light time (LT). The sham-exposed group showed a significant decrease in LT on the learning, consolidation, and retention days compared to other groups (p < 0.05). Pretreatment with vitamins (E and E + C) could protect PAL against adverse effects of RFW, and the administration of vitamin E + C improved PAL performance in control III compared to control I and treatment II groups (p < 0.05). Administration of vitamin E + C to exposed group (treatment II) caused a significant increase in LT on the learning (p = 0.013), consolidation, and retention (p = 0.009) sessions compared to the treatment group I (vitamin E). Long-term exposure to 900 MHz RFW impaired PAL and memory, and pretreatment of vitamin (E or E + C) prevented these effects, which may be a new potential mechanism against side effects of RFW.
Keywords: Electromagnetic field; learning and memory; passive avoidance performance; vitamin C; vitamin E.