Cost-effective culture of human induced pluripotent stem cells using UV/ozone-modified culture plastics with reduction of cell-adhesive matrix coating

Mater Sci Eng C Mater Biol Appl. 2020 Jun:111:110788. doi: 10.1016/j.msec.2020.110788. Epub 2020 Feb 29.


Human induced pluripotent stem cells (hiPSCs) are considered to be one of the most promising cell resources for regenerative medicine. HiPSCs usually maintain their pluripotency when they are cultured on feeder cell layers or are attached to a cell-adhesive extracellular matrix. In this study, we developed a culture system based on UV/ozone modification for conventional cell culture plastics to generate a suitable surface condition for hiPSCs. Time of flight secondary ion mass spectrometry (ToF-SIMS) was carried out to elucidate the relationship between hiPSC adhesion and UV/ozone irradiation-induced changes to surface chemistry of cell culture plastics. Cell culture plastics with modified surfaces enabled growth of a feeder-free hiPSC culture with markedly reduced cell-adhesive matrix coating. Our cell culture system using UV/ozone-modified cell culture plastics may produce clinically relevant hiPSCs at low costs, and can be easily scaled up in culture systems to produce a large number of hiPSCs.

Keywords: Cell adhesive matrix; Cell culture plastics; Human induced pluripotent stem cell; Surface chemistry; UV/ozone surface modification.

MeSH terms

  • Alkaline Phosphatase / metabolism
  • Cell Adhesion / drug effects
  • Cell Culture Techniques / economics*
  • Cell Proliferation / drug effects
  • Cell Proliferation / radiation effects
  • Cells, Cultured
  • Collagen / pharmacology
  • Cost-Benefit Analysis*
  • Drug Combinations
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / drug effects
  • Induced Pluripotent Stem Cells / radiation effects
  • Karyotype
  • Laminin / pharmacology
  • Ozone / pharmacology*
  • Plastics / pharmacology*
  • Pluripotent Stem Cells / cytology
  • Pluripotent Stem Cells / drug effects
  • Pluripotent Stem Cells / metabolism
  • Polystyrenes
  • Proteoglycans / pharmacology
  • Surface Properties
  • Ultraviolet Rays*


  • Drug Combinations
  • Laminin
  • Plastics
  • Polystyrenes
  • Proteoglycans
  • matrigel
  • Ozone
  • Collagen
  • Alkaline Phosphatase