Design and analysis of CRISPR-Cas experiments

Nat Biotechnol. 2020 Jul;38(7):813-823. doi: 10.1038/s41587-020-0490-7. Epub 2020 Apr 13.


A large and ever-expanding set of CRISPR-Cas systems now enables the rapid and flexible manipulation of genomes in both targeted and large-scale experiments. Numerous software tools and analytical methods have been developed for the design and analysis of CRISPR-Cas experiments, including resources to design optimal guide RNAs for various modes of manipulation and to analyze the results of such experiments. A major recent focus has been the development of comprehensive tools for use on data from large-scale CRISPR-based genetic screens. As this field continues to progress, a clear ongoing challenge is not only to innovate, but to actively maintain and improve existing tools so that researchers across disciplines can rely on a stable set of excellent computational resources for CRISPR-Cas experiments.

Publication types

  • Review

MeSH terms

  • CRISPR-Cas Systems / genetics*
  • Gene Editing*
  • Genetic Engineering / trends
  • Genome / genetics
  • Humans
  • RNA, Guide, Kinetoplastida / genetics*
  • Software*


  • RNA, Guide