Streptomyces soli sp. nov., isolated from birch forest soil

Arch Microbiol. 2020 Sep;202(7):1687-1692. doi: 10.1007/s00203-020-01878-z. Epub 2020 Apr 13.

Abstract

A Gram-staining positive, aerobic, non-motile, rod-shaped bacterium, designated strain LAM7114T, was isolated from soil sample collected from a birch forest in Xinjiang Uygur Autonomous Region, China. The optimal temperature and pH for the growth of strain LAM7114T were 30 °C and 7.0, respectively. Strain LAM7114T could grow in the presence of NaCl up to 10% (w/v). Comparative analysis of the 16S rRNA gene sequences revealed that LAM7114T was closely related to the members of the family genus Streptomyces, with the highest similarity to Streptomyces urticae NEAU-PCY-1T (98.3%) and Streptomyces fildesensis GW25-5T (98.2%). The genomic G + C content was 70.0 mol%. The DNA-DNA hybridization values between strain LAM7114T and S. urticae CCTCC AA 2017015T, S. fildesensis CGMCC 4.5735T were 32.5 ± 1.8% and 27.5 ± 2.6%, respectively. The cell wall contained LL-diaminopimelic acid as the diagnostic diamino acid in the peptidoglycan. The whole-cell hydrolysates included glucose and mannose. The major fatty acids were anteiso-C15:0, iso-C15:0 and iso-C16:0. The predominant menaquinones were MK-9(H6), MK-9(H4) and MK-9(H8). The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, three unidentified aminophospholipids, three unidentified phospholipids, and an unidentified aminolipid. Based on the phenotypic characteristics and genotypic analyses, we propose that strain LAM7114T represents a novel species in the genus Streptomyces, for which the name Streptomyces soli sp. nov. is proposed. The type strain is LAM7114T (= CGMCC 4.7581T = JCM 32822T).

Keywords: 16S rRNA gene; Polyphasic taxonomy; Soil; Streptomyces.

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • Betula / microbiology*
  • DNA, Bacterial / genetics
  • Diaminopimelic Acid / analysis
  • Fatty Acids / analysis
  • Forests*
  • Genotype
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Soil Microbiology*
  • Species Specificity
  • Streptomyces / classification*
  • Streptomyces / genetics
  • Streptomyces / isolation & purification

Substances

  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S
  • Diaminopimelic Acid