Simultaneous measurement of biochemical phenotypes and gene expression in single cells

Nucleic Acids Res. 2020 Jun 4;48(10):e59. doi: 10.1093/nar/gkaa240.


Methods to measure heterogeneity among cells are rapidly transforming our understanding of biology but are currently limited to molecular abundance measurements. We developed an approach to simultaneously measure biochemical activities and mRNA abundance in single cells to understand the heterogeneity of DNA repair activities across thousands of human lymphocytes, identifying known and novel cell-type-specific DNA repair phenotypes.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cell Line
  • DNA Repair*
  • Gene Expression*
  • Genomics
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Lymphocytes / metabolism
  • Phenotype
  • RNA, Messenger / chemistry
  • RNA, Messenger / metabolism
  • RNA-Seq
  • Sequence Analysis, DNA
  • Single-Cell Analysis / methods*


  • RNA, Messenger