Apamin from bee venom suppresses inflammation in a murine model of gouty arthritis

J Ethnopharmacol. 2020 Jul 15:257:112860. doi: 10.1016/j.jep.2020.112860. Epub 2020 Apr 11.

Abstract

Ethnopharmacological relevance: Bee venom (BV) has been used for the treatment of inflammatory diseases, such as rheumatoid arthritis, and for the relief of pain in traditional oriental medicine.

Aim of study: The aim of this study was to determine the anti-inflammatory effect of BV on monosodium urate (MSU)-induced gouty arthritis in a mouse model.

Materials and methods: To develop a mouse model of acute gouty arthritis, 4 mg 50 μL-1 of MSU crystal suspension was injected intradermally into the right paw. After MSU crystal injection, we evaluated inflammatory cytokine production in mice of the BV-treated (0.5 and 1 mg kg-1 body weight) and apamin (APM)-treated (0.5 and 1 mg kg-1 body weight) groups. The positive control group was administered a colchicine (1 mg kg-1 body weight) injection with MSU crystals.

Results: BV and APM treatment suppressed inflammatory paw edema in MSU-administered mice. It also exerted anti-inflammatory effects in mice with gouty arthritis by inhibiting proinflammatory cytokine production and inflammasome formation. Interestingly, MSU crystal formation was decreased by BV and APM treatment.

Conclusions: These results suggest that the APM from BV might be useful for the treatment of gouty arthritis due to its anti-inflammatory activities.

Keywords: Apamin; Bee venom; Gouty arthritis; Monosodium urate (MSU).

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Apamin / isolation & purification
  • Apamin / pharmacology*
  • Arthritis, Experimental / chemically induced
  • Arthritis, Experimental / drug therapy*
  • Arthritis, Experimental / metabolism
  • Arthritis, Experimental / pathology
  • Arthritis, Gouty / chemically induced
  • Arthritis, Gouty / metabolism
  • Arthritis, Gouty / pathology
  • Arthritis, Gouty / prevention & control*
  • Bee Venoms / chemistry
  • Bee Venoms / pharmacology*
  • Cytokines / genetics
  • Cytokines / metabolism
  • Inflammasomes / genetics
  • Inflammasomes / metabolism
  • Inflammation Mediators / metabolism
  • Joints / drug effects*
  • Joints / metabolism
  • Joints / pathology
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • RAW 264.7 Cells
  • Signal Transduction
  • Uric Acid

Substances

  • Anti-Inflammatory Agents
  • Bee Venoms
  • Cytokines
  • Inflammasomes
  • Inflammation Mediators
  • Apamin
  • Uric Acid