Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2

Emerg Infect Dis. 2020 Jul;26(7):1633-1635. doi: 10.3201/eid2607.201285. Epub 2020 Jun 21.


Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.

Keywords: 2019 novel coronavirus disease; COVID-19; SARS; SARS-CoV-2; coronavirus; coronavirus disease; molecular detection; rRT-PCR; respiratory infections; severe acute respiratory syndrome coronavirus 2; viruses; zoonoses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Betacoronavirus / genetics
  • Betacoronavirus / isolation & purification*
  • Humans
  • Multiplex Polymerase Chain Reaction / methods*
  • Nasopharynx / virology
  • Real-Time Polymerase Chain Reaction / methods*
  • SARS-CoV-2