Real-time RT-PCR in COVID-19 detection: issues affecting the results

doi:10.1080/14737159.2020.1757437

Editorial

. 2020 May;20(5):453-454.

doi: 10.1080/14737159.2020.1757437. Epub 2020 Apr 22.

Real-time RT-PCR in COVID-19 detection: issues affecting the results

Alireza Tahamtan¹, Abdollah Ardebili²

Affiliations

¹ Infectious Diseases Research Centre, Golestan University of Medical Sciences, Gorgan, Iran.
² Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.

PMID: 32297805
PMCID: PMC7189409
DOI: 10.1080/14737159.2020.1757437

Editorial

Real-time RT-PCR in COVID-19 detection: issues affecting the results

Alireza Tahamtan et al. Expert Rev Mol Diagn. 2020 May.

. 2020 May;20(5):453-454.

doi: 10.1080/14737159.2020.1757437. Epub 2020 Apr 22.

Authors

Alireza Tahamtan¹, Abdollah Ardebili²

Affiliations

¹ Infectious Diseases Research Centre, Golestan University of Medical Sciences, Gorgan, Iran.
² Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.

PMID: 32297805
PMCID: PMC7189409
DOI: 10.1080/14737159.2020.1757437

No abstract available

Keywords: COVID-19 detection; Real-time RT-PCR; SARS-CoV-2; false negative; false positive.

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References

1. Shen M, Zhou Y, Ye J, et al. Recent advances and perspectives of nucleic acid detection for coronavirus. J Pharm Anal. 2020. DOI:10.1016/j.jpha.2020.02.010. - DOI - PMC - PubMed
1. Wan Z, Zhang Y, He Z, et al. a melting curve-based multiplex RT-qPCR assay for simultaneous detection of four human coronaviruses. Int J Mol Sci. 2016. November 23;17(11):E1880. - PMC - PubMed
1. Noh JY, Yoon SW, Kim DJ, et al. Simultaneous detection of severe acute respiratory syndrome, middle east respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR. Arch Virol. 2017;162(6):1617–1623. - PMC - PubMed
1. Wang Y, Kang H, Liu X, et al. Combination of RT-qPCR testing and clinical features for diagnosis of COVID-19 facilitates management of SARS-CoV-2 outbreak. J Med Virol. 2020. February 25 [Epub ahead of print] DOI:10.1002/jmv.25721. - DOI - PMC - PubMed
1. Xi M, Wei Q, Qihua F, et al. Understanding the influence factors in viral nucleic acid test of 2019 novel coronavirus (2019-nCoV). Chin J Lab Med. 2020;43:E002–E002.

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[1] Shen M, Zhou Y, Ye J, et al. Recent advances and perspectives of nucleic acid detection for coronavirus. J Pharm Anal. 2020. DOI:10.1016/j.jpha.2020.02.010. - DOI - PMC - PubMed

[2] Shen M, Zhou Y, Ye J, et al. Recent advances and perspectives of nucleic acid detection for coronavirus. J Pharm Anal. 2020. DOI:10.1016/j.jpha.2020.02.010. - DOI - PMC - PubMed

[3] Wan Z, Zhang Y, He Z, et al. a melting curve-based multiplex RT-qPCR assay for simultaneous detection of four human coronaviruses. Int J Mol Sci. 2016. November 23;17(11):E1880. - PMC - PubMed

[4] Wan Z, Zhang Y, He Z, et al. a melting curve-based multiplex RT-qPCR assay for simultaneous detection of four human coronaviruses. Int J Mol Sci. 2016. November 23;17(11):E1880. - PMC - PubMed

[5] Noh JY, Yoon SW, Kim DJ, et al. Simultaneous detection of severe acute respiratory syndrome, middle east respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR. Arch Virol. 2017;162(6):1617–1623. - PMC - PubMed

[6] Noh JY, Yoon SW, Kim DJ, et al. Simultaneous detection of severe acute respiratory syndrome, middle east respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR. Arch Virol. 2017;162(6):1617–1623. - PMC - PubMed

[7] Wang Y, Kang H, Liu X, et al. Combination of RT-qPCR testing and clinical features for diagnosis of COVID-19 facilitates management of SARS-CoV-2 outbreak. J Med Virol. 2020. February 25 [Epub ahead of print] DOI:10.1002/jmv.25721. - DOI - PMC - PubMed

[8] Wang Y, Kang H, Liu X, et al. Combination of RT-qPCR testing and clinical features for diagnosis of COVID-19 facilitates management of SARS-CoV-2 outbreak. J Med Virol. 2020. February 25 [Epub ahead of print] DOI:10.1002/jmv.25721. - DOI - PMC - PubMed

[9] Xi M, Wei Q, Qihua F, et al. Understanding the influence factors in viral nucleic acid test of 2019 novel coronavirus (2019-nCoV). Chin J Lab Med. 2020;43:E002–E002.

[10] Xi M, Wei Q, Qihua F, et al. Understanding the influence factors in viral nucleic acid test of 2019 novel coronavirus (2019-nCoV). Chin J Lab Med. 2020;43:E002–E002.

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Real-time RT-PCR in COVID-19 detection: issues affecting the results

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Real-time RT-PCR in COVID-19 detection: issues affecting the results

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