Pantothenic acid promotes dermal papilla cell proliferation in hair follicles of American minks via inhibitor of DNA Binding 3/Notch signaling pathway

Life Sci. 2020 Jul 1:252:117667. doi: 10.1016/j.lfs.2020.117667. Epub 2020 Apr 15.


Aims: Pantothenic acid (PA) has been applied to treat alopecia, but the underlying mechanism is still unclear. Our study aims to explore the underlying mechanism of PA in regulating hair follicle (HF) growth.

Main methods: Mink HFs and dermal papilla (DP) cells were isolated and cultured in vitro. HFs and DP cells were treated with 0, 10, 20, 40 μg/ml PA. The effect of PA on HF growth, DP cell proliferation, cell cycle distribution, cell migration, and insulin-like growth factor-1 (IGF-1) and vascular endothelial growth factor (VEGF) expressions in DP cells was measured. Moreover, the effect of PA on inhibitor of DNA binding 3 (ID3)/Notch signaling pathway was analyzed. Subsequently, ID3 was silenced to validate whether ID3/Notch signaling pathway was involved in regulating DP cell proliferation by PA.

Key findings: Both 20 μg/ml and 40 μg/ml PA promoted HF growth, G1/S transition of DP cells and IGF-1 and VEGF expressions in DP cells, while only 20 μg/ml PA promoted cell viability and the migration of DP cells. Thus 20 μg/ml PA was chosen for the following experiments. PA treatment was found to up-regulate ID3 expression but down-regulate Notch receptor 1 (Notch1) and Notch signaling targets expressions. Furthermore, ID3 knockdown reversed PA-induced cell proliferation and inhibition of Notch1 and Notch signaling targets expressions, indicating that PA-induced DP cell proliferation and inhibition of Notch signaling were mediated via up-regulation of ID3.

Significance: This study provides an underlying mechanism related to the effect of PA on stimulating DP cell proliferation.

Keywords: Dermal papilla cell; Hair follicle; ID3; Notch; Pantothenic acid.

MeSH terms

  • Animals
  • Cell Movement / drug effects
  • Cell Proliferation / drug effects*
  • Cell Survival / drug effects
  • Dermis / cytology
  • Dermis / drug effects*
  • Dose-Response Relationship, Drug
  • Gene Expression Regulation / drug effects
  • Hair Follicle / cytology
  • Hair Follicle / drug effects*
  • Inhibitor of Differentiation Proteins / metabolism
  • Male
  • Mink
  • Pantothenic Acid / administration & dosage
  • Pantothenic Acid / pharmacology*
  • Receptors, Notch / metabolism
  • Signal Transduction / drug effects


  • Inhibitor of Differentiation Proteins
  • Receptors, Notch
  • Pantothenic Acid