Dendrigraft poly-L-lysines delivery of DNA vaccine effectively enhances the immunogenic responses against H9N2 avian influenza virus infection in chickens

Kai Zhao; Guangyu Rong; Qiaoyang Teng; Xuesong Li; Hailing Lan; Lu Yu; Shuang Yu; Zheng Jin; Guangping Chen; Zejun Li

doi:10.1016/j.nano.2020.102209

Dendrigraft poly-L-lysines delivery of DNA vaccine effectively enhances the immunogenic responses against H9N2 avian influenza virus infection in chickens

Nanomedicine. 2020 Jul:27:102209. doi: 10.1016/j.nano.2020.102209. Epub 2020 Apr 16.

Authors

Kai Zhao¹, Guangyu Rong², Qiaoyang Teng³, Xuesong Li³, Hailing Lan², Lu Yu⁴, Shuang Yu⁴, Zheng Jin⁵, Guangping Chen⁶, Zejun Li⁷

Affiliations

¹ Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin, China; Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Science, Heilongjiang University, Harbin, China. Electronic address: zybin395@126.com.
² Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin, China; Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Science, Heilongjiang University, Harbin, China; Department of Avian Infectious Disease, and Innovation Team for Pathogenic Ecology Research on Animal Influenza, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai, China.
³ Department of Avian Infectious Disease, and Innovation Team for Pathogenic Ecology Research on Animal Influenza, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai, China.
⁴ Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin, China; Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Science, Heilongjiang University, Harbin, China.
⁵ Key Laboratory of Chemical Engineering Process and Technology for High-efficiency Conversion, College of Chemistry and Material Sciences, Heilongjiang University, Harbin, China.
⁶ Department of Physiological Sciences, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK, USA. Electronic address: guangping.chen@okstate.edu.
⁷ Department of Avian Infectious Disease, and Innovation Team for Pathogenic Ecology Research on Animal Influenza, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai, China. Electronic address: lizejun@shvri.ac.cn.

PMID: 32305593
DOI: 10.1016/j.nano.2020.102209

Abstract

Biodegradable nanomaterials can protect antigens from degradation, promote cellular absorption, and enhance immune responses. We constructed a eukaryotic plasmid [pCAGGS-opti441-hemagglutinin (HA)] by inserting the optimized HA gene fragment of H9N2 AIV into the pCAGGS vector. The pCAGGS-opti441-HA/DGL was developed through packaging the pCAGGS-opti441-HA with dendrigraft poly-l-lysines (DGLs). DGL not only protected the pCAGGS-opti441-HA from degradation, but also exhibited high transfection efficiency. Strong cellular immune responses were induced in chickens immunized with the pCAGGS-opti441-HA/DGL. The levels of IFN-γ and IL-2, and lymphocyte transformation rate of the vaccinated chickens increased at the third week post the immunization. For the vaccinated chickens, T lymphocytes were activated and proliferated, the numbers of CD3+CD4+ and CD4+/CD8+ increased, and the chickens were protected completely against H9N2 AIV challenge. This study provides a method for the development of novel AIV vaccines, and a theoretical basis for the development of safe and efficient gene delivery carriers.

Keywords: Delivery of DNA vaccine; Dendrigraft poly-L-lysine; H9N2 avian influenza; Immune responses; Nanoparticles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Viral / immunology*
Antibodies, Viral / pharmacology
Antibody Formation / drug effects
Antibody Formation / immunology
Chickens / immunology
Chickens / virology
Influenza A Virus, H9N2 Subtype / drug effects
Influenza A Virus, H9N2 Subtype / immunology
Influenza A Virus, H9N2 Subtype / pathogenicity
Influenza Vaccines / chemistry
Influenza Vaccines / genetics
Influenza Vaccines / immunology
Influenza Vaccines / pharmacology*
Influenza in Birds / drug therapy*
Influenza in Birds / immunology
Influenza in Birds / virology
Polylysine / chemistry
Polylysine / pharmacology
Vaccines, DNA / chemistry
Vaccines, DNA / immunology
Vaccines, DNA / pharmacology*

Substances

Antibodies, Viral
Influenza Vaccines
Vaccines, DNA
Polylysine