A Bioassay for Determining Symbiotic Zooxanthellae Shape Control Using Lectin SLL-2 from the Octocoral Sinularia lochmodes

Mitsuru Jimbo; Ryota Takeuchi; Mayu Yoshino

doi:10.1007/978-1-0716-0430-4_36

A Bioassay for Determining Symbiotic Zooxanthellae Shape Control Using Lectin SLL-2 from the Octocoral Sinularia lochmodes

Methods Mol Biol. 2020:2132:369-378. doi: 10.1007/978-1-0716-0430-4_36.

Authors

Mitsuru Jimbo¹, Ryota Takeuchi^{2

3}, Mayu Yoshino²

Affiliations

¹ Department of Marine Biosciences, Kitasato University, Sagamihara, Kanagawa, Japan. mjinbo@kitasato-u.ac.jp.
² Department of Marine Biosciences, Kitasato University, Sagamihara, Kanagawa, Japan.
³ AIST-Osaka University Advanced Photonics and Biosensing Open Innovation Laboratory, AIST, Photonics Center Osaka University P3, Suita, Osaka, Japan.

PMID: 32306344
DOI: 10.1007/978-1-0716-0430-4_36

Abstract

Symbiosis with zooxanthellae is essential for survival of corals. Using a bioassay, we report the H-type lectin SLL-2 purified from the octocoral Sinularia lochmodes to restrict zooxanthellae form to spherical cells. However, the factor for initiating or maintaining a symbiotic relationship between a host and zooxanthellae has not been found in many corals. This bioassay is useful for evaluating the role of a lectin as a symbiosis-related factor.

Keywords: Bioassay; SLL-2; Symbiosis; Transformation; Zooxanthella.

MeSH terms

Animals
Anthozoa / metabolism
Anthozoa / parasitology*
Biological Assay
Dinoflagellida / drug effects*
Dinoflagellida / physiology
Lectins / isolation & purification
Lectins / pharmacology*
Symbiosis

Substances

Lectins