Effect of drugs in vitro on lysosomal enzyme activities in bovine retinal pigment epithelial cells

Jpn J Ophthalmol. 1988;32(3):316-21.


We examined in vitro the effect of several drugs on the activities of lysosomal enzymes in the crude extract of bovine retinal pigment epithelial cells. Acid phosphatase, beta-D-glucuronidase, N-acetyl-beta-D-glucosaminidase, alpha-L-fucosidase, and alpha-D-mannosidase were used as lysosomal enzymes. Sodium iodate at 10(-6), 10(-5) and 10(-4) M, and potassium iodate at 10(-5) and 10(-4) M inhibited acid phosphatase activity. Ferrous chloride at 10(-5) and 10(-4) M suppressed beta-D-glucuronidase activity. Ferric chloride, indomethacin, chloroquine, chlorpromazine, 5-fluorouracil, tobramycin, daunomycin, oxalate and epinephrine had no or only minimal inhibitory effects on the lysosomal enzyme activities examined.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Ferrous Compounds / pharmacology
  • In Vitro Techniques
  • Iodates / pharmacology
  • Lysosomes / enzymology*
  • Pigment Epithelium of Eye / drug effects*
  • Pigment Epithelium of Eye / enzymology
  • Potassium / pharmacology
  • Potassium Compounds*


  • Ferrous Compounds
  • Iodates
  • Potassium Compounds
  • potassium iodate
  • Potassium
  • ferrous chloride
  • sodium iodate