Effects of hyperbaric oxygen therapy on the expression levels of the inflammatory factors interleukin-12p40, macrophage inflammatory protein-1β, platelet-derived growth factor-BB, and interleukin-1 receptor antagonist in keloids

Medicine (Baltimore). 2020 Apr;99(16):e19857. doi: 10.1097/MD.0000000000019857.


Background: Our study aimed to screen and explore the expression of inflammatory factors in keloid patients and to investigate how hyperbaric oxygen (HBO) therapy affects the expression levels of interleukin-12p40 (IL-12p40), macrophage inflammatory protein-1β (MIP-1β), platelet-derived growth factor-BB (PDGF-BB), and interleukin-1 receptor antagonist (IL-1Ra).

Objective: 30 patients were randomly selected and divided into the following 3 groups: keloid samples from keloid patients treated with HBO therapy (A), keloid samples from keloid patients treated without HBO therapy (B), and normal control skin samples derived from individuals who had no clear scarring (C). Each group included 10 samples.

Methods: Inflammatory factors in the keloid tissues were measured with the MILLIPLEX multiplexed Luminex system. Hematoxylin and eosin staining, immunohistochemical staining, and Western blotting were used to observe the morphological differences in different tissues and the expression levels.

Results: The expression levels of inflammatory mediators, including IL-12p40, MIP-1β, PDGF-BB, and IL-1Ra, in keloid tissues were significantly different from those in samples of normal skin. Hematoxylin and eosin staining showed significantly greater inflammatory infiltration in keloid tissue. Significantly different expression levels were observed in group A, B, and C.

Conclusion: Significantly altered levels of inflammatory factors in the samples from keloid patients were observed, suggesting that formation of a keloid is potentially related to inflammatory responses. HBO therapy could significantly affect the expression levels of IL-12p40, MIP-1β, PDGF-BB, and IL-1Ra, indicating that the effects of HBO therapy are associated with the attenuation of inflammatory responses.

Publication types

  • Clinical Trial
  • Comparative Study

MeSH terms

  • Adult
  • Becaplermin / metabolism*
  • Chemokine CCL4 / metabolism*
  • Female
  • Humans
  • Hyperbaric Oxygenation / adverse effects*
  • Hyperbaric Oxygenation / methods
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-12 Receptor beta 1 Subunit / metabolism*
  • Keloid / metabolism
  • Keloid / pathology
  • Keloid / therapy*
  • Male
  • Middle Aged
  • Receptors, Interleukin-1 / antagonists & inhibitors


  • CCL4 protein, human
  • Chemokine CCL4
  • IL1RN protein, human
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-12 Receptor beta 1 Subunit
  • Receptors, Interleukin-1
  • Becaplermin