Pelargonidin suppresses adipogenesis in 3T3-L1 cells through inhibition of PPAR-γ signaling pathway

Arch Biochem Biophys. 2020 Jun 15;686:108365. doi: 10.1016/j.abb.2020.108365. Epub 2020 Apr 18.

Abstract

Pelargonidin is a natural compound that exists widely in fruits, and exerts antioxidant, anti-atherosclerotic, anti-inflammatory, anti-hyperglycemic, and anti-diabetic activities. However, there have not been any studies concerning its anti-obesity potential to date. Therefore, we evaluated the anti-obesity potential of pelargonidin via inhibition of adipogenesis in 3T3-L1 cells. The cellular oil droplet content was decreased to 68.14%, 56.75%, and 48.39% and triglyceride accumulation decreased to 74.53%, 61.54%, and 47.86% after incubation with 5 μM, 10 μM, and 20 μM pelargonidin, respectively, when compared with DMSO group. Furthermore, pelargonidin treatment led to decrease in glucose consumption. Western blot assay illustrated that the expression of PPAR-γ was suppressed to 63.25%, 47.52%, and 21.23% after incubation with 5 μM, 10 μM, and 20 μM pelargonidin when compared with DMSO group. Then, we measured the expression of some target proteins of PPAR-γ, and found that pelargonidin decreased the expressions of HMGCR, LPL, Glut4, and A-FABP. Besides, the result of Luciferase Reporter Assay indicated that pelargonidin inhibited PPAR-γ transcription activity. These results indicated that pelargonidin exerts anti-adipogenic activity in 3T3-L1 cells through inhibition of PPAR-γ signaling pathway, and pelargonidin could be used as a potential anti-obesity agent.

Keywords: 3T3-L1 cells; Adipogenesis; PPAR-γ; Pelargonidin; Triglyceride.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Adipogenesis / drug effects*
  • Animals
  • Anthocyanins / metabolism
  • Anthocyanins / pharmacology*
  • Anti-Obesity Agents / metabolism
  • Anti-Obesity Agents / pharmacology*
  • Down-Regulation / drug effects
  • Fatty Acid-Binding Proteins / genetics
  • Fatty Acid-Binding Proteins / metabolism
  • Gene Expression Regulation / drug effects
  • Glucose / metabolism
  • Glucose Transporter Type 4 / genetics
  • Glucose Transporter Type 4 / metabolism
  • Lipoprotein Lipase / genetics
  • Lipoprotein Lipase / metabolism
  • Mice
  • PPAR gamma / metabolism*
  • Triglycerides / genetics
  • Triglycerides / metabolism

Substances

  • Anthocyanins
  • Anti-Obesity Agents
  • Fatty Acid-Binding Proteins
  • Glucose Transporter Type 4
  • PPAR gamma
  • Triglycerides
  • pelargonidin
  • Lipoprotein Lipase
  • Glucose