3'-Hydroxypterostilbene Potently Alleviates Obesity Exacerbated Colitis in Mice

J Agric Food Chem. 2020 May 13;68(19):5365-5374. doi: 10.1021/acs.jafc.0c01782. Epub 2020 Apr 30.

Abstract

Epidemiological surveys show that obesity and the western diet increase the risk of colitis. Studies have also confirmed that the high-fat-diet (HFD) promoted the deterioration of colitis-related indicators in mice. Compared with stilbenoids, the results showed that 3'-hydroxypterostilbene (HPSB) was found to be the most effective inhibitor for the antiadipogenesis and anti-inflammation. However, its role in ameliorating obesity-promoted colitis is still unknown. We intend to investigate the protective effect and related molecular mechanisms of HPSB on HFD promoted dextran sodium sulfate (DSS)-induced colitis in mice. The results indicate that colitis in the HFD+DSS group tends to be more apparent in the DSS-only group, while feeding 0.025% of HPSB at different stages can improve the colitis induced by HFD+DSS. HPSB significantly reduced the levels of interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) induced by HFD+DSS in mice. Furthermore, the Western blotting revealed that the administration of HPSB significantly downregulated cyclooxygenase-2 (COX-2), plasmalemma vesicle-associated protein-1 (PV-1), and phospho-signal transducer and activator of transcription 3 (p-STAT3) expressions in HFD+DSS treated mice. Presented results reveal that HPSB is a novel functional agent capable of preventing HFD exacerbated colitis.

Keywords: 3′-hydroxypterostilbene (HPSB); chemoprevention; colitis; obesity.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / administration & dosage
  • Chemokine CCL2 / genetics
  • Chemokine CCL2 / immunology
  • Colitis / chemically induced
  • Colitis / drug therapy*
  • Colitis / genetics
  • Colitis / immunology
  • Dextran Sulfate / adverse effects
  • Diet, High-Fat / adverse effects
  • Disease Models, Animal
  • Humans
  • Interleukin-6 / genetics
  • Interleukin-6 / immunology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Obesity / complications*
  • Obesity / etiology
  • Obesity / genetics
  • Obesity / immunology
  • STAT3 Transcription Factor / genetics
  • STAT3 Transcription Factor / immunology
  • Stilbenes / administration & dosage*

Substances

  • 3'-hydroxypterostilbene
  • Anti-Inflammatory Agents
  • Chemokine CCL2
  • Interleukin-6
  • STAT3 Transcription Factor
  • Stilbenes
  • Dextran Sulfate