Background: The incidence and mortality of lung cancer often rank first in all malignant tumors. DNA methylation, as one of epigenetics, often participates in the development and progression of tumors. CDO1 as a tumor suppressor gene always undergoes methylation changes early in tumor development. Therefore, this study aims to discuss the value of CDO1 methylation in the early diagnosis of lung cancer.
Methods: Peripheral blood samples were collected from tumor patients and healthy people. Detection of the methylation level of CDO1 in plasma by sulfite modification and quantitative real-time PCR.
Results: The level of gene methylation in peripheral blood of lung cancer patients was significantly higher than that of benign lung disease patients and healthy people. The methylation level of CDO1 was significantly different in the stratified comparison of gender, lymph node metastasis and tumor-node-metastasis (TNM) stage (P<0.05). The sensitivity and specificity of CDO1 were 52.2% and 78.6%, respectively. The overall accuracy of the diagnosis was significantly higher than that of the clinical tumor markers, and the sensitivity of CDO1 to stage I and II patients was the highest (40.8%, 47.1%). In addition, CDO1 could effectively increase the sensitivity of diagnosis in multiple joint examinations.
Conclusions: Detecting the methylation level of CDO1 has a potentially huge advantage for the early diagnosis of lung cancer.
【中文题目：血浆中CDO1甲基化在肺癌早期诊断中的作用研究】 【中文摘要：背景与目的 肺癌的发生率和死亡率常居所有恶性肿瘤的首位，DNA甲基化作为表观遗传学之一参与肿瘤的发生发展过程，CDO1作为抑癌基因常在肿瘤发生早期便会发生甲基化改变，因此本研究旨在探讨CDO1甲基化在肺癌早期诊断中的价值。方法 收集肿瘤患者和健康人群的外周血液样本，游离DNA通过亚硫酸盐修饰并结合实时荧光定量PCR检测CDO1在外周血中的甲基化水平。结果 肺癌患者的外周血的基因甲基化水平明显高于肺部良性疾病患者及健康人群。肺癌患者CDO1的甲基化水平在性别、淋巴结转移和肿瘤原发灶-淋巴结-转移（tumor-node-metastasis, TNM）分期的分层比较中存在显著性差异（P<0.05）。CDO1对肺癌诊断的灵敏度和特异性分别为52.2%和78.6%。其诊断的整体准确度明显高于应用于临床的肿瘤标志物而且对I期、II期患者的诊断灵敏度表现最好（40.8%, 47.1%）。此外，CDO1可有效增加多项联检中诊断的灵敏性。结论 检测CDO1的甲基化水平对肺癌的早期诊断具有潜在的巨大优势。】 【中文关键词：半胱氨酸双加氧酶1；甲基化；DNA；肺肿瘤；血浆】.
Keywords: CDO1; DNA; Lung neoplasms; Methylation; Plasma.