Multi-Infection Patterns and Co-infection Preference of 27 Human Papillomavirus Types Among 137,943 Gynecological Outpatients Across China

Guangdong Liao; Xiyi Jiang; Bin She; Huijuan Tang; Zhongyong Wang; Hongrong Zhou; Yan Ma; Weidong Xu; Hongxing Xu; Wen Chen; Jianguang Ji; Mingrong Xi; Tianhui Chen

doi:10.3389/fonc.2020.00449

Multi-Infection Patterns and Co-infection Preference of 27 Human Papillomavirus Types Among 137,943 Gynecological Outpatients Across China

Front Oncol. 2020 Apr 7:10:449. doi: 10.3389/fonc.2020.00449. eCollection 2020.

Authors

Guangdong Liao^{1

2}, Xiyi Jiang³, Bin She⁴, Huijuan Tang³, Zhongyong Wang⁵, Hongrong Zhou⁶, Yan Ma⁷, Weidong Xu⁸, Hongxing Xu⁸, Wen Chen⁹, Jianguang Ji¹⁰, Mingrong Xi^{1

2}, Tianhui Chen^{11

12

13}

Affiliations

¹ Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu, China.
² Key Laboratory of Birth Defects and Related Diseases of Women and Children Affiliated to the Ministry of Education, Sichuan University, Chengdu, China.
³ Institute of Occupational Diseases, Hangzhou Medical College, Hangzhou, China.
⁴ Department of Academic Development, Tellgen Corporation, Shanghai, China.
⁵ Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Zhejiang, China.
⁶ Department of Gynecology and Obstetrics, Changning Maternity and Infant Health Hospital, Shanghai, China.
⁷ Department of Gynecology and Obstetrics, The First Affiliated Hospital of University of South China, Hengyang, China.
⁸ Department of Clinical Laboratory, Suzhou Municipal Hospital, Suzhou, China.
⁹ Department of Cancer Epidemiology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
¹⁰ Center for Primary Health Care Research, Lund University, Lund, Sweden.
¹¹ Institute of Cancer and Basic Medicine (ICBM), Chinese Academy of Sciences (CAS), Hangzhou, China.
¹² Department of Cancer Prevention, Cancer Hospital of the University of CAS (Zhejiang Cancer Hospital), Hangzhou, China.
¹³ Zhejiang Provincial Office for Cancer Prevention and Control, Zhejiang Cancer Center, Hangzhou, China.

Abstract

Background: The epidemiological feature of human papillomavirus (HPV) infection is distinctive in China. We aimed to investigate the multi-infection patterns and co-infection preference of 27 HPV types among gynecological outpatients across China. Methods: Overall 137,943 gynecological outpatients were recruited from eight tertiary hospitals located in seven regions of China, between July 1st, 2014 and December 31st, 2016. The overall, region-specific, age-specific and type-specific prevalence of HPV infection were calculated, respectively. The pattern of HPV infection was also evaluated. Furthermore, rate ratio was calculated to evaluate the co-infection preference of any two HPV genotypes. Results: The overall prevalence of 27 HPVs' [17 high-risk (hr)/10 low-risk (lr)] infection was 23.5%. The age-specific HPV prevalence showed a "U-shaped" pattern. The most prevalent hrHPV genotypes were 16, 52, and 58. Multiple infections were detected in 25.8% of the HPV-positive women, in which dual infection was more prevalent. HPV 16/18 were likely to co-infected with HPV 31 but unlikely with HPV 52/58, i.e., the co-infection of HPV 16 with HPV 31 was high (3.5-fold), but low for HPV 58 (1.8-fold), and 52 (1.2-fold), while the co-infection of HPV 18 with HPV 31 was high (4.3-fold), but low for HPV 52 (1.9-fold), and 58 (1.7-fold). Conclusions: We found age-specific prevalence of HPV infection showed a "U-shaped" pattern for high and low risk HPV, suggesting the importance of screening among younger women and the necessary of detection among older women. We found a novel co-infection preference of HPV 16/18 with 31, 52, and 58, suggesting a need of developing and marketing prophylactic HPV vaccines that protect against more genotypes in China.

Keywords: cervical cancer; co-infection; human papillomavirus; prevalence; vaccine.