Anti-SARS-CoV-2 Virus Antibody Levels in Convalescent Plasma of Six Donors Who Have Recovered From COVID-19

Aging (Albany NY). 2020 Apr 22;12(8):6536-6542. doi: 10.18632/aging.103102. Epub 2020 Apr 22.

Abstract

Background: Anti-SARS-CoV-2 virus antibody levels in convalescent plasma (CP), which may be useful in severe Anti-SARS-CoV-2 virus infections, have been rarely reported.

Results: A total of eight donors were considered for enrollment; two of them were excluded because of ineligible routine check. Of the six remaining participants, five samples were tested weakly positive by the IgM ELISA. Meanwhile, high titers of IgG were observed in five samples. The patient treated with CP did not require mechanical ventilation 11 days after plasma transfusion, and was then transferred to a general ward.

Conclusions: Our serological findings in convalescent plasma from recovered patients may help facilitate understanding of the SARS-CoV-2 infection and establish CP donor screening protocol in COVID-19 outbreak.

Methods: Anti-SARS-CoV-2 antibodies including IgM and IgG were measured by two enzyme-linked immunosorbent assays (ELISA) in convalescent plasma from six donors who have recovered from coronavirus disease 2019 (COVID-19) in Nanjing, China. CP was also utilized for the treatment of one severe COVID-19 patient.

Keywords: COVID-19; SARS-CoV-2 virus; anti-SARS-CoV-2 antibodies; convalescent plasma; coronavirus disease 2019; plasma donation.

MeSH terms

  • Antibodies, Viral / blood*
  • Betacoronavirus / immunology*
  • Blood Donors*
  • Coronavirus Infections / blood*
  • Coronavirus Infections / immunology
  • Coronavirus Infections / therapy
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Immunization, Passive
  • Immunoglobulin G / blood
  • Immunoglobulin M / blood
  • Pandemics
  • Pneumonia, Viral / blood*
  • Pneumonia, Viral / immunology
  • Pneumonia, Viral / therapy

Substances

  • Antibodies, Viral
  • Immunoglobulin G
  • Immunoglobulin M

Supplementary concepts

  • COVID-19
  • COVID-19 serotherapy
  • severe acute respiratory syndrome coronavirus 2