Biomaterials for orthopedics: anti-biofilm activity of a new bioactive glass coating on titanium implants

Biofouling. 2020 Feb;36(2):234-244. doi: 10.1080/08927014.2020.1755842. Epub 2020 Apr 22.


This study evaluated adhesion and biofilm formation by Candida albicans, Pseudomonas aeruginosa and Staphylococcus epidermidis on surfaces of titanium (Ti) and titanium coated with F18 Bioactive Glass (BGF18). Biofilms were grown and the areas coated with biofilm were determined after 2, 4 and 8 h. Microscopy techniques were applied in order to visualize the structure of the mature biofilm and the extracellular matrix. On the BGF18 specimens, there was less biofilm formation by C. albicans and S. epidermidis after incubation for 8 h. For P. aeruginosa biofilm, a reduction was observed after incubation for 4 h, and it remained reduced after 8 h on BGF18 specimens. All biofilm matrices seemed to be thicker on BGF18 surface than on titanium surfaces. BGF18 showed significant anti-biofilm activity in comparison with Ti in the initial periods of biofilm formation; however, there was extensive biofilm after incubation for 48 h.

Keywords: Bioactive glass; Biofilms; Candida albicans; Pseudomonas aeruginosa; Staphylococcus epidermidis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biocompatible Materials / chemistry*
  • Biofilms / growth & development*
  • Candida albicans / growth & development
  • Glass / chemistry*
  • Prostheses and Implants / microbiology*
  • Pseudomonas aeruginosa / growth & development
  • Staphylococcus epidermidis / growth & development
  • Surface Properties
  • Titanium / chemistry*


  • Biocompatible Materials
  • Titanium