Adriamycin interactions with T4 DNA polymerase. Two modes of template-mediated inhibition

J Biol Chem. 1977 Apr 25;252(8):2670-4.


We examined the effect of adriamycin on kinetics of DNA synthesis catalyzed by DNA polymerase purified from bacteriophage T4-infected Escherichia coli. Two distinct modes of enzyme inhibition occur: uncompetitive and competitive at "low" and "high" drug:DNA nucleotide molar ratios, respectively. Competitive inhibition is not observed unless an unblocked amino group is present on the sugar (daunosamine) moiety. A model is proposed to relate the enzyme inhibition kinetics to intercalative and ionic binding of adriamycin to DNA.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Coliphages / enzymology*
  • DNA-Directed DNA Polymerase / metabolism*
  • Doxorubicin / analogs & derivatives
  • Doxorubicin / pharmacology*
  • Escherichia coli / enzymology
  • Kinetics
  • Structure-Activity Relationship
  • Templates, Genetic


  • Doxorubicin
  • DNA-Directed DNA Polymerase