A potential downstream platform approach for WuXiBody-based IgG-like bispecific antibodies

Protein Expr Purif. 2020 Sep:173:105647. doi: 10.1016/j.pep.2020.105647. Epub 2020 Apr 23.


WuXiBody is a novel bispecific antibody (bsAb) platform developed by WuXi Biologics. It enables almost any monoclonal antibody (mAb) sequence pair to be assembled into a bispecific construct. BsAbs based on WuXiBody can adopt either asymmetric or symmetric format. WuXiBody's unique design not only ensures desired chain pairing but also facilitates removal of potential product-related impurities. In this work, demonstrated with four WuXiBody-based bsAbs (two asymmetric and two symmetric ones), we showed that Protein A followed by anion exchange (AEX) and mixed-mode chromatography (i.e., Capto MMC ImpRes or Capto adhere ImpRes) can be a potential platform approach for WuXiBody purification.

Keywords: Bispecific antibody (bsAb); Homodimer; Knobs-into-holes (KiH); Mixed-mode chromatography; T cell receptor (TCR); WuXiBody.

MeSH terms

  • Animals
  • Antibodies, Bispecific* / biosynthesis
  • Antibodies, Bispecific* / chemistry
  • Antibodies, Bispecific* / genetics
  • Antibodies, Bispecific* / isolation & purification
  • CHO Cells
  • Cricetulus
  • Humans
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / isolation & purification


  • Antibodies, Bispecific
  • Recombinant Proteins