Investigation of the uptake and transport of polysaccharide from Se-enriched Grifola frondosa in Caco-2 cells model

Qingfang Xiang; Weijie Zhang; Qian Li; Jie Zhao; Weiwei Feng; Ting Zhao; Guanghua Mao; Yao Chen; Xiangyang Wu; Liuqing Yang; Guangying Chen

doi:10.1016/j.ijbiomac.2020.04.160

Investigation of the uptake and transport of polysaccharide from Se-enriched Grifola frondosa in Caco-2 cells model

Int J Biol Macromol. 2020 Apr 24:S0141-8130(20)33021-X. doi: 10.1016/j.ijbiomac.2020.04.160. Online ahead of print.

Authors

Qingfang Xiang¹, Weijie Zhang², Qian Li³, Jie Zhao¹, Weiwei Feng⁴, Ting Zhao¹, Guanghua Mao⁴, Yao Chen⁴, Xiangyang Wu⁴, Liuqing Yang⁵, Guangying Chen⁶

Affiliations

¹ School of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.
² School of Food and Biological Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.
³ School of Food Science and Engineering, Yangzhou University, Huayangxi Rd. 196, Yangzhou 225127, Jiangsu, China.
⁴ School of the Environment and Safety Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, Jiangsu, China.
⁵ School of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China. Electronic address: yangliuqing@ujs.edu.cn.
⁶ Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, Hainan Normal University, Longkun Rd. 99, Hainan 570100, China. Electronic address: chgying123@163.com.

PMID: 32339585
DOI: 10.1016/j.ijbiomac.2020.04.160

Abstract

A variety of beneficial pharmacological activities have been reported for Se-enriched Grifola frondosa polysaccharides. However, little has been reported on its absorption, and its intestinal uptake and transport profiles remain unknown. Based on our previous research, the aim of this study was to investigate its absorption from two aspects - the polysaccharides and selenium of Se-enriched Grifola frondosa polysaccharides (Se-GFP-22) across Caco-2 cells in vitro. The Caco-2 cells monolayer culture model was successfully constructed to study the transport and uptake of Se-GFP-22. The results revealed that the uptake and transport of Se-GFP-22 were time- and concentration- dependent. Transport studies illustrated that Se-GFP-22 could penetrate Caco-2 cells, mainly mediated through the same routes as endocytosis and selenium in the organic selenium (Se-GFP-22) was more easily absorbed than that in the inorganic selenium control group (sodium selenite). The uptake of Se-GFP-22 may be a macropinocytosis pathway, which was an accumulation from cytoplasm to nucleus process. Se-GFP-22 was a moderately absorbed biological macromolecule testified by the apparent permeability coefficients (Papp) value and transport rates. This work illustrates the characteristics on uptake and transport of Se-GFP-22 and all these results may help to explore the mechanism of polysaccharide absorption in vitro.

Keywords: Caco-2 cells; Organic selenium; Se-enriched Grifola frondosa; Selenium polysaccharide; Transport and uptake.