In ovo injection of nano-selenium (Se) produced by lactic acid bacteria (LAB-nano-Se) was investigated on the hatchability, immune responses and the histopathological alterations in hatched chicks. The eggs (18 day age) were injected with 0.5 ml of 0.9% NaCl (normal saline, NS), while the control group was kept without injection. In the third, fourth and fifth groups, the eggs were injected with 0.5 ml of NS and LAB-nano-Se at 10, 20 and 30 μg/egg. The results revealed improved growth performance in groups injected with LAB-nano-Se when compared to the control treatment. The highest final weight and weight gain were noticed in 20 μg LAB-nano-Se/egg group (p < .05). The feed conversion ratio was reduced in all treated groups when compared to the control group (p < .05). Groups injected with LAB-nano-Se showed enhanced hatchability of the whole incubated eggs (p < .05). Total lipids and cholesterol levels were decreased significantly in groups treated with LAB-nano-Se at 10 and 20 μg/egg when compared to the non-treated group. At the same time, globulin was increased by LAB-nano-Se in ovo injection. Furthermore, the total antioxidant capacity, catalase, glutathione peroxidase, superoxide dismutase increased in groups treated with LAB-nano-Se at 10 and 20 μg/egg with insignificant (p > .05) differences with those treated with LAB-nano-Se at 30 μg/egg using in ovo injection technique. Also, higher total blood protein and phagocytosis were significantly observed in groups treated with at 10, 20 and 30 μg LAB-nano-Se/egg. The histopathological images of hatched chicks revealed that nano-Se presented normal effects on liver and kidney tissues and restored the parameters as mentioned earlier. To conclude, LAB-nano-Se exhibited beneficial effects in hatched chicks through improving immune and antioxidant activities as well as histopathological effects by using in ovo technique.
Keywords: LAB-nano-selenium; hatchability; histopathology; immunity; in ovo injection; oxidative status.
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