Isolation and purification of acidic polysaccharides from Agaricus blazei Murill and evaluation of their lipid-lowering mechanism

Int J Biol Macromol. 2020 Aug 15:157:276-287. doi: 10.1016/j.ijbiomac.2020.04.190. Epub 2020 Apr 25.


Polysaccharides are important active constituents of Agaricus blazei Morrill. In the present study, WABM-A was isolated from WABM using DEAE-cellulose, and subsequently purified using sepharose CL-6B to obtain the acidic polysaccharide WABM-A-b. WABM-A-b is mainly composed of Glc dextran, with a molecular weight of 10 KDa and β-1,6-D-Glcp as its main chain. The results of in vivo experiments show that in comparison with the MG, WABM-A significantly reduced the serum levels of TC, TG, and LDL-C, increased the serum levels of HDL-C (P < 0.01), and upregulated the liver expression of PPARγ, LXRα, ABCA1, and ABCG1 in rats with hyperlipidemia (P < 0.05). The results of in vitro experiments show that in comparison with the MG group, WABM-A-b-H significantly reduced the levels of TC and TG in HepG2 cells induced by oleic acid (P < 0.01), and significantly upregulated the protein expression of PPARγ, LXRα, ABCA1, and ABCG1 (P < 0.05). The present study demonstrates that WABM-A-b is an acidic glucan with lipid-lowering activity. The lipid-lowering mechanism of WABM-A-b is via the activation of the PPARγ/LXRα/ABCA1/ABCG1 cholesterol metabolism pathway. This is the first time that the hypolipidemic effect of Agaricus blazei Morrill acidic polysaccharides has been reported.

Keywords: Agaricus blazei Murrill; Lipid-lowering; Polysaccharide.

MeSH terms

  • Agaricus / chemistry*
  • Animals
  • Cell Survival
  • Disease Models, Animal
  • Glucans
  • Hep G2 Cells
  • Humans
  • Hyperlipidemias / drug therapy
  • Lipid Metabolism / drug effects*
  • Lipids / blood*
  • Liver / metabolism*
  • Liver / pathology
  • Male
  • Oleic Acid
  • Polysaccharides / isolation & purification*
  • Polysaccharides / pharmacology*
  • Rats
  • Rats, Sprague-Dawley
  • Signal Transduction / drug effects
  • Up-Regulation / drug effects


  • Glucans
  • Lipids
  • Polysaccharides
  • Oleic Acid

Supplementary concepts

  • Agaricus blazei