During the migration of cancer cells for metastasis, cancer cells can be exposed to fluid shear conditions. We examined two breast cancer cell lines, MDA-MB-468 (less metastatic) and MDA-MB-231 (more metastatic), and a benign MCF-10A epithelial cell line for their responsiveness in migration to fluid shear. We tested fluid shear at 15 dyne/cm2 that can be encountered during breast cancer cells travelling through blood vessels or metastasizing to mechanically active tissues such as bone. MCF-10A exhibited the least migration with a trend of migrating in the flow direction. Intriguingly, fluid shear played a potent role as a trigger for MDA-MB-231 cell migration, inducing directional migration along the flow with significantly increased displacement length and migration speed and decreased arrest coefficient relative to unflowed MDA-MB-231. In contrast, MDA-MB-468 cells were markedly less migratory than MDA-MB-231 cells, and responded very poorly to fluid shear. As a result, MDA-MB-468 cells did not exhibit noticeable difference in migration between static and flow conditions, as was distinct in root mean square (RMS) displacement - an ensemble average of all participating cells. These may suggest that the difference between more metastatic MDA-MB-231 and less metastatic MDA-MB-468 breast cancer cells could be at least partly involved with their differential responsiveness to fluid shear stimulatory cues. Our study provides new data in regard to potential crosstalk between fluid shear and metastatic potential in mediating breast cancer cell migration.
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P2Y2 receptor activation by nucleotides released from highly metastatic breast cancer cells increases tumor growth and invasion via crosstalk with endothelial cells.Breast Cancer Res. 2014 Aug 26;16(5):R77. doi: 10.1186/bcr3694. Breast Cancer Res. 2014. PMID: 25156554 Free PMC article.
Roles for GP IIb/IIIa and αvβ3 integrins in MDA-MB-231 cell invasion and shear flow-induced cancer cell mechanotransduction.Cancer Lett. 2014 Mar 1;344(1):62-73. doi: 10.1016/j.canlet.2013.10.019. Epub 2013 Oct 28. Cancer Lett. 2014. PMID: 24176823
The effect of soluble E-selectin on tumor progression and metastasis.BMC Cancer. 2016 May 24;16:331. doi: 10.1186/s12885-016-2366-2. BMC Cancer. 2016. PMID: 27220365 Free PMC article.
Specific expression of the human voltage-gated proton channel Hv1 in highly metastatic breast cancer cells, promotes tumor progression and metastasis.Biochem Biophys Res Commun. 2011 Aug 26;412(2):353-9. doi: 10.1016/j.bbrc.2011.07.102. Epub 2011 Jul 29. Biochem Biophys Res Commun. 2011. PMID: 21821008
Parathyroid hormone-related protein and bone metastases.Cancer. 1997 Oct 15;80(8 Suppl):1572-80. doi: 10.1002/(sici)1097-0142(19971015)80:8+<1572::aid-cncr7>3.3.co;2-d. Cancer. 1997. PMID: 9362424 Review.