Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 Alleviate Escherichia coli- Induced depression and Gut Dysbiosis in Mice

J Microbiol Biotechnol. 2020 Aug 28;30(8):1222-1226. doi: 10.4014/jmb.2002.02058.

Abstract

Lactobacillus reuteri NK33 (NK33) and Bifidobacterium adolescentis NK98 (NK98) alleviate immobilization stress-induced depression. To understand the gut microbiota-mediated mechanisms of NK33 and NK98 against depression, we examined their effects on Escherichia coli K1 (K1)-induced depression and gut dysbiosis in mice. NK33, NK98, and their mixtures (1:1, 4:1, and 9:1) mitigated K1-induced depression and colitis. NK33 and NK98 additively or synergistically increased BDNF+/NeuN+ cell population and suppressed NF-κB action in the hippocampus. They alleviated gut dysbiosis by reducing the Proteobacteria population and increasing the Clostridia population. These results suggest that NK33 and NK98 may alleviate depression and colitis by ameliorating gut dysbiosis.

Keywords: Bifidobacterium adolescentis; Lactobacillus reuteri; depression; gut microbiota.

MeSH terms

  • Animals
  • Bifidobacterium adolescentis / physiology*
  • Brain-Derived Neurotrophic Factor / genetics
  • Brain-Derived Neurotrophic Factor / metabolism
  • Colitis / microbiology
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Depression / microbiology
  • Depression / therapy*
  • Disease Models, Animal
  • Dysbiosis / microbiology
  • Dysbiosis / therapy*
  • Escherichia coli / pathogenicity*
  • Feces / microbiology
  • Gastrointestinal Microbiome / physiology*
  • Lactobacillus reuteri / physiology*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism

Substances

  • Bdnf protein, mouse
  • Brain-Derived Neurotrophic Factor
  • DNA-Binding Proteins
  • Nerve Tissue Proteins
  • NeuN protein, mouse