Modular and Versatile trans-Encoded Genetic Switches

Angew Chem Int Ed Engl. 2020 Apr 30. doi: 10.1002/anie.202001372. Online ahead of print.


Current bacterial RNA switches suffer from lack of versatile inputs and are difficult to engineer. Here we present versatile and modular RNA switches that are trans -encoded and based on tRNA mimicking structures (TMSs). These switches provide a high degree of freedom for reengineering and can thus be designed to accept a wide range of inputs including RNA, small molecules and proteins. This powerful approach allows controlling translation of protein expression from plasmid and genome DNA.

Keywords: RNA Switch; RNA, protein and small molecule inputs; logic gate; tRNA mimicking structure.