Modular and Versatile Trans-Encoded Genetic Switches

Angew Chem Int Ed Engl. 2020 Nov 9;59(46):20328-20332. doi: 10.1002/anie.202001372. Epub 2020 Jul 27.


Current bacterial RNA switches suffer from lack of versatile inputs and are difficult to engineer. We present versatile and modular RNA switches that are trans-encoded and based on tRNA-mimicking structures (TMSs). These switches provide a high degree of freedom for reengineering and can thus be designed to accept a wide range of inputs, including RNA, small molecules, and proteins. This powerful approach enables control of the translation of protein expression from plasmid and genome DNA.

Keywords: RNA; RNA logic gates; RNA switches; synthetic biology; tRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gene Expression
  • Green Fluorescent Proteins / genetics
  • RNA, Bacterial / genetics*
  • Synthetic Biology


  • RNA, Bacterial
  • Green Fluorescent Proteins