Current bacterial RNA switches suffer from lack of versatile inputs and are difficult to engineer. Here we present versatile and modular RNA switches that are trans -encoded and based on tRNA mimicking structures (TMSs). These switches provide a high degree of freedom for reengineering and can thus be designed to accept a wide range of inputs including RNA, small molecules and proteins. This powerful approach allows controlling translation of protein expression from plasmid and genome DNA.
Keywords: RNA Switch; RNA, protein and small molecule inputs; logic gate; tRNA mimicking structure.
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