Modular and Versatile trans-Encoded Genetic Switches

Angew Chem Int Ed Engl. 2020 Apr 30. doi: 10.1002/anie.202001372. Online ahead of print.

Abstract

Current bacterial RNA switches suffer from lack of versatile inputs and are difficult to engineer. Here we present versatile and modular RNA switches that are trans -encoded and based on tRNA mimicking structures (TMSs). These switches provide a high degree of freedom for reengineering and can thus be designed to accept a wide range of inputs including RNA, small molecules and proteins. This powerful approach allows controlling translation of protein expression from plasmid and genome DNA.

Keywords: RNA Switch; RNA, protein and small molecule inputs; logic gate; tRNA mimicking structure.