Genome Sequence Comparison of Staphylococcus aureus TX0117 and a Beta-Lactamase-Cured Derivative Shows Increased Cationic Peptide Resistance Accompanying Mutations in relA and mnaA

Mia Jade Sales; George Sakoulas; Richard Szubin; Bernhard Palsson; Cesar Arias; Kavindra V Singh; Barbara E Murray; Jonathan M Monk

doi:10.1128/MRA.01515-19

Genome Sequence Comparison of Staphylococcus aureus TX0117 and a Beta-Lactamase-Cured Derivative Shows Increased Cationic Peptide Resistance Accompanying Mutations in relA and mnaA

Microbiol Resour Announc. 2020 Apr 30;9(18):e01515-19. doi: 10.1128/MRA.01515-19.

Authors

Mia Jade Sales¹, George Sakoulas², Richard Szubin¹, Bernhard Palsson^{1

2}, Cesar Arias^{3

4}, Kavindra V Singh^{3

4}, Barbara E Murray^{3

4}, Jonathan M Monk⁵

Affiliations

¹ Bioengineering Department, University of California San Diego, La Jolla, California, USA.
² Department of Pediatrics, University of California, San Diego School of Medicine, La Jolla, California, USA.
³ Division of Infectious Diseases, Department of Internal Medicine, McGovern Medical School at the University of Texas Health Science Center at Houston (UTHealth), Houston, Texas, USA.
⁴ Center for Antimicrobial Resistance and Microbial Genomics, McGovern Medical School at the University of Texas Health Science Center at Houston (UTHealth), Houston, Texas, USA.
⁵ Bioengineering Department, University of California San Diego, La Jolla, California, USA jmonk@ucsd.edu.

Abstract

Staphylococcus aureus strain TX0117 is a methicillin-susceptible bacterium with type A beta-lactamase exhibiting a high cefazolin inoculum effect. TX0117 was cured of blaZ, yielding TX0117c with increased antimicrobial peptide resistance. The sequencing and genome assembly of TX0117 elucidate six mutations between TX0117 and TX0117c, including relA truncation and mnA_1 substitution.

Grants and funding

U01 AI124316/AI/NIAID NIH HHS/United States